Highly Efficient Genome Modifications Mediated by CRISPR/Cas9 in Drosophila
Zhongsheng Yu, Mengda Ren, Zhanxiang Wang, Bo Zhang, Yikang S. Rong, Renjie Jiao, Guanjun Gao


We report that Cas9/gRNA mediates efficient genetic modifications in Drosophila. Through targeting seven loci, we achieved a germline efficiency of up to 100%. Genes in both heterochromatin and euchromatin can be modified efficiently. Thus the Cas9/gRNA system is an attractive tool for rapid disruption of essentially any gene in Drosophila.

  • Received May 31, 2013.
  • Accepted June 28, 2013.
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