Autophagy is an evolutionarily conserved degradative pathway that has been implicated in a number of physiological events important for human health. This process was originally identified as a response to nutrient deprivation and is thought to serve in a recycling capacity during periods of nutritional stress. Autophagy activity appears to be highly regulated and multiple signaling pathways are known to target a complex of proteins that contains the Atg1 protein kinase. The data here extend these observations and identify a particular phosphorylation event on Atg1 as a potential control point within the autophagy pathway in Saccharomyces cerevisiae. This phosphorylation occurs at a threonine residue, T226, within the Atg1 activation loop that is conserved in all Atg1 orthologs. Replacing this threonine with a nonphosphorylatable residue resulted in a loss of Atg1 protein kinase activity and a failure to induce autophagy. This phosphorylation required the presence of a functional Atg1 kinase domain and two known regulators of Atg1 activity, Atg13 and Atg17. Interestingly, the levels of this modification were found to increase dramatically upon exposure to conditions that induce autophagy. In addition, T226 phosphorylation was associated with an autophosphorylated form of Atg1 that was found specifically in cells undergoing the autophagy process. In all, these data suggest that autophosphorylation within the Atg1 activation loop may represent a point of regulatory control for this degradative process.
MACROAUTOPHAGY (hereafter referred to as autophagy) is a highly conserved process of self-degradation that is essential for cell survival during periods of nutrient limitation (Tsukada and Ohsumi 1993). During autophagy, a double membrane grows out from a specific nucleation site, known as the pre-autophagosomal structure, or PAS, in Saccharomyces cerevisiae and the phagophore assembly site in mammals (Suzuki and Ohsumi 2007). This membrane encapsulates bulk protein and other constituents of the cytoplasm and ultimately targets this material to the vacuole/lysosome for degradation (Xie and Klionsky 2007). Recent studies have linked this pathway to a number of processes important for human health, including tumor suppression, innate immunity, and neurological disorders, like Huntington's disease (Rubinsztein et al. 2007; Levine and Kroemer 2008). Determining how this pathway is regulated is therefore important for our understanding of these processes and our attempts to manipulate autophagy in clinically beneficial ways.
Most of the molecular components of the autophagy pathway were initially characterized in the budding yeast, S. cerevisiae, but orthologs of many of these Atg proteins have since been found in other eukaryotes (Tsukada and Ohsumi 1993; Meijer et al. 2007). A complex of proteins that contains the Atg1 protein kinase is of special interest and appears to be a key point of regulatory control within this pathway (Kamada et al. 2000; Budovskaya et al. 2005; He and Klionsky 2009; Stephan et al. 2009). In S. cerevisiae, genetic and biochemical data indicate that this complex is targeted by at least three different signaling pathways. Two of these pathways, involving the Tor and cAMP-dependent protein kinases, inhibit this process, whereas the AMP-activated protein kinase is needed for the full induction of autophagy (Noda and Ohsumi 1998; Wang et al. 2001; Budovskaya et al. 2004; Stephan and Herman 2006; Kamada et al. 2010). The manner in which these signaling pathways regulate Atg1 activity and the precise role of this kinase in the autophagy process are presently matters of intense scrutiny.
Although Atg1 kinase activity is required for the induction of autophagy, relatively little is known about how this enzyme is regulated in vivo. Two proteins associated with Atg1, Atg13 and Atg17, have been shown to be required for full Atg1 kinase activity both in vitro and in vivo (Kamada et al. 2000; Stephan et al. 2009). The roles of these proteins appear to be conserved through evolution as functional homologs of both have been identified in fruit flies and/or mammals (Hara et al. 2008; Chan et al. 2009; Chang and Neufeld 2009; Ganley et al. 2009; Hosokawa et al. 2009; Jung et al. 2009; Mercer et al. 2009). However, it is not yet clear precisely how these proteins stimulate Atg1 activity. In this study, we show that Atg1 is autophosphorylated within the activation loop and that this phosphorylation is required for both Atg1 kinase activity and the induction of autophagy. The activation loop is a structurally conserved element within the kinase domain and phosphorylation within this loop is often a necessary prerequisite for efficient substrate binding and/or phosphotransfer in the catalytic site (Johnson et al. 1996; Nolen et al. 2004). This loop generally corresponds to the sequence between two signature elements within the core kinase domain, the DFG and APE motifs (Hanks and Hunter 1995). Phosphorylation within this loop tends to result in a more ordered structure for this region and the proper positioning of key elements within the catalytic core of the kinase domain (Knighton et al. 1991; Johnson and O'reilly 1996; Huse and Kuriyan 2002). We found that Atg1 activation loop phosphorylation was correlated with the onset of autophagy and that replacing the site of phosphorylation with a phosphomimetic residue led to constitutive Atg1 autophosphorylation in vivo. In all, the data here suggest that Atg1 phosphorylation within its activation loop may be an important point of regulation within the autophagy pathway and models that discuss these data are presented.
MATERIALS AND METHODS
Strains and growth media:
Standard Escherichia coli growth conditions and media were used throughout this study. The yeast rich-growth medium, YPAD, consists of 1% yeast extract, 2% Bacto-peptone, 500 mg/liter adenine–HCl, and 2% glucose. The yeast YM glucose and SC glucose minimal growth media have been described (Kaiser et al. 1994; Chang et al. 2001). The nitrogen starvation medium, SD-N, consists of 0.17% yeast nitrogen base lacking amino acids and ammonium sulfate, and 2% glucose. Growth media reagents were from Difco (Detroit). Standard yeast genetic methods were used in this study. The yeast strains used were TN125 (MATa ade2 his3 leu2 lys2 trp1 ura3 pho8∷pho8Δ60), YYK126 (TN125 atg1Δ∷LEU2), YYK130 (TN125 atg13Δ∷TRP1), PHY3687 (TN125 atg1Δ∷LEU2 atg13Δ∷kanMX), BY4741 (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0), PHY4167 (BY4741 atg1Δ∷kanMX), PHY4177 (BY4741 atg13Δ∷kanMX), and PHY4181 (BY4741 atg17Δ∷kanMX) (Noda et al. 1995; Kamada et al. 2000; Giaever et al. 2002).
Site-directed mutageneses were performed with the Clontech Transformer kit on the ATG1-containing plasmids, pPHY1115 and pPHY2376, to generate the variants (K54A, D211A, T226A, and T226E) used herein. Plasmid pPHY1115 was originally named pRS316-3xmycATG1 and was generously provided by Dr. Yoshinori Ohsumi. Plasmid pPHY2376 is a pRS423-based construct in which Atg1 has three copies of the HA epitope at its N terminus; this plasmid was originally provided by Dr. Daniel Klionsky. Variants in the YFP–Atg1 fusion background were generated similarly with the wild-type plasmid, pPHY2297, as the starting vehicle (Budovskaya et al. 2005). For the rAtg1 variants, fragments encoding residues 1–420 were PCR amplified from the appropriate yeast plasmids and cloned into the pET21a expression vector. The plasmid, pPHY2427, encoded an HA epitope-tagged version of Atg13 under the control of the promoter from the copper-inducible CUP1 gene. The ATG13 locus was cloned from the plasmid, pRS316–ATG13, that was kindly provided by Dr. Takeshi Noda. A site-directed mutagenesis was performed to insert an XmaI site immediately following the start codon of ATG13. The ATG13 coding sequences and transcriptional terminator were then cloned as a 3.1 kb XmaI–NotI fragment into pPHY2203 to form pPHY2427. The pPHY2203 plasmid was described previously and contains the CUP1 promoter and the HA epitope (Deminoff et al. 2006).
Western blotting and immunoprecipitation:
Protein samples for Western blotting were prepared as described (Budovskaya et al. 2005). The proteins were separated on SDS–polyacrylamide gels, transferred to nitrocellulose membranes and the membranes were probed with the appropriate primary and secondary antibodies. The Supersignal chemiluminescent substrate (Pierce Chemical, Rockford, IL) or Odyssey infrared imaging system (LI-COR Biosciences) was subsequently used to detect the reactive bands or fluorescence intensities. Immunoprecipitations were performed as described (Budovskaya et al. 2005; Deminoff et al. 2009). HA-tagged proteins were generally precipitated on anti-HA matrix beads (Roche) whereas myc-tagged proteins were immunoprecipitated with a monoclonal anti-myc antibody and then collected on Protein A–Sepharose (GE Healthcare) as described (Budovskaya et al. 2005; Deminoff et al. 2006). The amount of immunoprecipitated and co-immunoprecipitated proteins were subsequently assessed by Western immunoblotting with the appropriate antibodies. To assess phosphorylation of position T226 within Atg1, a polyclonal antiserum was generated in rabbits against a phosphorylated peptide, FLPNTSLAE(pT)LCGSPLY, that corresponds to the sequence of the Atg1 activation loop (see Figure 1A). The peptide synthesis, and antibody generation and purification was performed by Lampire Biological Laboratories. Western blots with this antibody were generally performed with cells overexpressing Atg1 from a 2μ plasmid.
In vitro kinase assays (IVKAs):
The immunoprecipitated Atg1 proteins, or purified recombinant Atg1 fragments, were incubated for 30–60 min at 30° with 10 μCi [γ-32P]ATP in a 40-μl reaction (50 mm potassium phosphate, 5 mm NaF, 10 mm MgCl2, 4.5 mm DTT, protease inhibitors, and phosphatase inhibitors) with or without 10 μg of myelin basic protein (MBP) (Sigma, St. Louis). The reactions products were separated on SDS–polyacrylamide gels and the gels were fixed, dried, and analyzed either by autoradiography or by phosphorimaging with a Typhoon Trio (GE Healthcare).
Recombinant protein purification:
E. coli cells were grown to an OD600 of ∼0.5/ml and IPTG was added to a final concentration of 1 mm to induce expression of the rAtg1 proteins. The induction was carried out for 4 hr. The cells were collected by centrifugation and lysed by sonication in lysis buffer (50 mm sodium phosphate, 500 mm NaCl, and protease inhibitors). Clarified cell lysates were then incubated with 1 ml Ni–NTA agarose beads (Qiagen) at 4° overnight. The beads were collected by centrifugation and washed three times with PBS containing 20 mm imidazole, and the recombinant proteins were eluted with PBS containing 250 mm imidazole.
Autophagy activity was assessed with several different assays that have been described previously. In general, autophagy was induced by treating mid-log-phase cells with 200 ng/ml rapamycin for the indicated times. The ALP-based assay measures the delivery and subsequent activation in the vacuole of an altered form of the Pho8 alkaline phosphatase, known as Pho8Δ60 (Noda et al. 1995; Noda and Klionsky 2008). The relative level of autophagy was indicated by the difference in alkaline phosphatase activity detected between extracts prepared from log-phase and rapamycin-treated cells. The data presented represent the average of at least three independent experiments where the autophagy levels generally varied by less than 15% between the different experimental replicates. In the GFP–Atg8 assay, the relative level of free GFP generated by proteolysis in the vacuole is used as an indicator of autophagy activity (Shintani and Klionsky 2004). The GFP–Atg8 fusion protein and the free GFP were detected by Western blotting with an anti-GFP antibody. The final assay assesses the processing of overexpressed aminopeptidase I, or Ape1, that occurs via the autophagy pathway (Budovskaya et al. 2004). In this assay, the cells express Ape1 at a level that saturated the cytoplasm-to-vacuole, or Cvt, transport system. Previous work has shown that the precursor Ape1 protein in these cells is processed by the autophagy pathway following either rapamycin treatment or nitrogen starvation (Budovskaya et al. 2004). The Ape1 proteins were detected by Western blotting with an anti-Ape1 antibody provided by Dr. Daniel Klionsky.
The CFP–Atg11 and YFP–Atg1 fusions were under the control of the inducible promoter from the yeast CUP1 gene. Expression of the fusion proteins was induced by the addition of 100 μm CuSO4 for 2 hr at 30°, and the samples were imaged as described (Budovskaya et al. 2005).
Residue T226 within the activation loop was a site of Atg1 autophosphorylation:
The activation loop of the S. cerevisiae Atg1 contains a candidate site of phosphorylation at threonine-226 (T226). This position, and much of the surrounding sequence, is conserved in Atg1 orthologs (Figure 1A). To test whether T226 was indeed a site of phosphorylation, we generated an antibody that specifically recognized the phosphorylated form of a peptide whose sequence corresponded to that of the Atg1 activation loop (Figure 1A; see materials and methods). This antibody recognized the wild-type Atg1 protein but not the Atg1T226A and Atg1T226E variants that had substitutions at position T226 (Figure 1B). Interestingly, the signal with this antibody was found to increase upon exposure to conditions that lead to an induction of autophagy, such as rapamycin treatment (Figure 1B; see below). The recognition by this antibody was lost upon phosphatase treatment of the immunoprecipitated Atg1 and was absent from cells expressing only the kinase-defective variants, Atg1K54A or Atg1D211A (Figure 1, C and D). The positions altered in these variants, K54 and D211, are highly conserved in protein kinases and are important for the proper positioning of ATP within the active site (Zoller et al. 1981; Hanks et al. 1988; Adams 2001). These results suggested that the recognition by this antibody required the presence of a phosphate group at position T226 and that the addition of this phosphate might be catalyzed by Atg1 itself. To examine this latter possibility further, we attempted to regenerate the phosphorylation signal at T226 in vitro with immunoprecipitated Atg1 proteins that had been pretreated with λ phosphatase. We found that the T226 phosphorylation was restored with the wild-type Atg1 but not with kinase-defective variants (Figure 1E; data not shown). Altogether, these data suggested that position T226 within the activation loop was a site of Atg1 autophosphorylation and that the presence of this modification was coincident with the induction of autophagy.
Phosphorylation at position T226 within the activation loop was required for Atg1 protein kinase activity:
To assess the importance of activation loop phosphorylation for Atg1 function, we replaced T226 with a nonphosphorylatable alanine residue and assessed Atg1 kinase activity both in vivo and in vitro. The in vitro assays examined Atg1 autophosphorylation and the phosphorylation of a nonphysiological substrate, the MBP (Matsuura et al. 1997; Kamada et al. 2000). In each case, the Atg1 proteins examined were precipitated from rapamycin-treated cells. We found that this Atg1T226A variant exhibited significantly diminished activity, relative to the wild-type enzyme, in both of these assays (Figure 2, A–D). Two kinase-defective versions of Atg1, Atg1K54A and Atg1D211A, were used as negative controls in these experiments and the level of kinase activity associated with Atg1T226A was similar to that observed with these control proteins. We also found that a pre-incubation of the precipitated Atg1 with ATP resulted in elevated kinase activity toward MBP (Figure 2C). This stimulation required the presence of the kinase domain and suggested that Atg1 autophosphorylation might increase the specific activity of this enzyme (supporting information, Figure S1, A and B). This autophosphorylation could occur at position T226 and/or other positions within the Atg1 protein.
To assess Atg1 kinase activity in vivo, we took advantage of previous observations indicating that Atg1 autophosphorylation results in the production of a slower-migrating form of this protein on SDS-polyacrylamide gels (Atg1-P in Figure 3A) (Kamada et al. 2000; Budovskaya et al. 2005; Stephan et al. 2009). Our data indicate that the site of autophosphorylation responsible for this shift is distinct from T226 (see below and our unpublished observations). The relative level of this second, upper band can serve as a measure of the Atg1 kinase activity present in cells (Stephan et al. 2009). Consistent with the in vitro data above, we found significantly less of the Atg1T226A variant, relative to the wild type, in the slower-migrating form following either rapamycin treatment or nitrogen starvation, two conditions that are known to induce an autophagy response (Figure 3, A and B). The relative activity of the Atg1T226A variant again resembled the kinase-defective controls, Atg1K54A and Atg1D211A. Interestingly, we found that the α-T226P antibody specifically recognized this slower-migrating form of Atg1 on Western blots (Figure 3, B and C). In all, these data suggested that T226 phosphorylation within the activation loop was required for Atg1 kinase activity and was associated with an autophosphorylated form of Atg1 found specifically in cells undergoing autophagy.
Activation loop phosphorylation was required for the induction of autophagy:
We used three different assays to test whether phosphorylation within the Atg1 activation loop was required for the induction of autophagy. The first assessed the vacuolar delivery and processing of an altered form of the Pho8 alkaline phosphatase, known as Pho8Δ60 (Noda et al. 1995). This truncated protein lacks a membrane-spanning domain and the targeting information necessary for normal delivery through the secretory pathway (Klionsky and Emr 1989; Noda et al. 1995). As a result, this protein can be delivered to the vacuole, and therein activated, only by the autophagy pathway. Using this assay, we found that no significant autophagy activity was detectable in cells carrying only the Atg1T226A variant (Figure 4A). The levels of autophagy detected were similar to those associated with the atg1Δ control strain.
The second assay measured the processing of a GFP–Atg8 fusion protein (Shintani and Klionsky 2004). Atg8 is normally incorporated into the autophagosome and delivered into the vacuolar lumen (Kim et al. 2001; Suzuki et al. 2001; Suzuki and Ohsumi 2007). The Atg8 is then degraded by vacuolar hydrolases, thereby releasing the more stable GFP protein (Shintani and Klionsky 2004). The relative level of free GFP can be used as a measure of the autophagy activity present. As with the ALP-based assay, no significant processing of the GFP–Atg8 fusion was detected in cells with the Atg1T226A variant (Figure 4B). This autophagy defect was corroborated by an examination of the subcellular localization of the GFP fluorescence in these cells. In wild-type cells, the GFP–Atg8 fusion is delivered to the vacuole and the free GFP accumulates within the lumen of this organelle (Figure 5). In contrast, very little, if any, GFP fluorescence is detected in the vacuoles of mutants defective for autophagy (see atg1Δ in Figure 4) (Suzuki et al. 2001). Consistent with the above processing defects, we found no significant GFP fluorescence within the vacuoles of atg1Δ cells carrying the Atg1T226A variant (Figure 5). It should be noted that this GFP–Atg8 fusion was normally localized to the PAS in these cells (Figure 5).
Finally, we assessed the autophagy-dependent maturation of theApe1 protein. Ape1 is a normal cargo for the cytoplasm-to-vacuole, or Cvt, transport pathway that is responsible for delivering a set of proteins to the vacuole during log-phase growth (Klionsky et al. 1992; Khalfan and Klionsky 2002). Overexpression of Ape1 saturates the Cvt system and results in the cytoplasmic accumulation of an unprocessed, precursor form of this protein (see Figure 4C) (Klionsky et al. 1992). Upon the induction of autophagy, this protein is efficiently delivered to the vacuole and processed to its mature, active form (Scott et al. 1996; Budovskaya et al. 2004). We found that the Atg1T226A variant was defective for this autophagy-mediated processing and that the Ape1 protein was present only in its precursor form in these cells (Figure 4C). Altogether, these data indicated that Atg1 autophosphorylation at T226 was necessary for the induction of the autophagy pathway in S. cerevisiae.
The Atg1T226E variant exhibited constitutive autophosphorylation activity:
To test whether the presence of T226 phosphorylation was sufficient for Atg1 autophosphorylation, we replaced this position with the potential phosphomimetic residues, glutamic and aspartic acid. Similar results were obtained with both substitutions, and we focus here on the Atg1T226E variant. Interestingly, we found that this Atg1T226E variant was competent for autophosphorylation in vitro when isolated from rapamycin-treated cells (Figure S2, A). However, unlike the wild-type protein, Atg1T226E also exhibited elevated levels of autophosphorylation in log-phase cells that were typically not undergoing autophagy. This constitutive, or unregulated, autophosphorylation activity was observed in both in vitro and in vivo assays (Figure 6, A–C). For example, in an in vitro assay, elevated levels of radioactivity, relative to the wild-type protein, were incorporated into the Atg1T226E variant isolated from log-phase cells (Figure 6A). In addition, the fraction of this variant found in the slower-migrating form on SDS-polyacrylamide gels was elevated in log-phase cell extracts (Figure 6, B and C). In both assays, the overall level of autophosphorylation was slightly elevated with the Atg1T226E variant. These in vivo results with the Atg1T226E protein supported the above contention that the presence of the slower-migrating form of Atg1 was due to autophosphorylation at a site distinct from T226. In particular, we found that the upper band associated with the Atg1T226E protein was lost upon phosphatase treatment and was regenerated in a subsequent incubation with ATP (Figure 6D and Figure S2, B).
Finally, we found that a recombinant fragment of Atg1 produced in E. coli (rAtg1) was also capable of catalyzing an autophosphorylation reaction (Figure 7). This fragment contained residues 1–420 of the wild-type protein and included the entire protein kinase domain located at the N terminus of Atg1. This autophosphorylation was also dependent upon the presence of T226 as replacing this position with an alanine residue resulted in significantly diminished activity (Figure 7). This rAtg1T226A variant also exhibited decreased activity in assays with MBP as substrate (Figure S3). In contrast, a recombinant protein with glutamic acid replacing T226 was capable of significant autophosphorylation (Figure 7). This result was similar to that obtained above with the wild-type protein and together these observations suggested that it was the phosphorylation at position T226 that was important for this Atg1 autophosphorylation. Moreover, the presence of a phosphomimetic at position 226 appeared to disrupt the normal control of Atg1 and resulted in constitutive autophosphorylation.
The Atg1T226E variant was nonfunctional for autophagy:
We also tested whether the Atg1T226E and Atg1T226D variants were functional for autophagy. Using the three processing assays described above, we found little, if any, autophagy activity in atg1Δ cells carrying these variants (Figure 8, A–C). Moreover, we found that the GFP–Atg8 fusion protein was not transported efficiently into the lumen of the vacuole in atg1–T226E cells (Figure 8D). This latter defect is consistent with a failure to carry out the normal autophagy process. Therefore, although these variants exhibited constitutive autophosphorylation activity, neither was capable of replacing the wild-type protein in the autophagy process. These variants also failed to induce autophagy in log-phase cells and had little detrimental effect upon autophagy when overexpressed in wild-type cells (Figure 8A and Figure S4). The autophagy defects did not appear to be due simply to gross misfolding as the Atg1T226E protein was present at normal steady-state levels in yeast cells, was localized normally to the PAS, and interacted with both Atg13 and Atg17, two key regulators of Atg1 kinase activity (Figure 9, A–C) (Kamada et al. 2000; Kabeya et al. 2005). However, we did find that the Atg1T226E variant was less effective than the wild-type protein at phosphorylating the nonphysiological substrate, MBP, in an in vitro assay (Figure 9D). Therefore, it is possible that the autophagy defects observed with this variant might be due to a failure to phosphorylate an important, but as yet unknown, substrate in vivo.
Atg13 and Atg17 were required for T226 phosphorylation:
The precise roles of Atg13 and Atg17 in the regulation of Atg1 kinase activity have not yet been defined. Here, we tested whether these regulators were required for the phosphorylation of Atg1 within its activation loop. Indeed, we found that T226 phosphorylation was greatly diminished in both atg13Δ and atg17Δ cells (Figure 10A). The residue levels remaining in these mutants were higher than those observed in strains carrying only a kinase-defective version of Atg1 (Figure S5). This low level of T226 phosphorylation might be important for the suppression of atg13 defects observed upon the overexpression of Atg1 in these mutants (Funakoshi et al. 1997). We also found that the overexpression of Atg13 resulted in an elevated level of T226 phosphorylation in both log-phase and rapamycin-treated cells (Figure 10B). Consistent with this observation, the overexpression of Atg13 also led to slightly increased levels of autophagy in rapamycin-treated cultures (Figure 10C). Finally, we tested whether the autophosphorylation associated with the Atg1T226E variant required either Atg13 or Atg17. We found that the slower-migrating form of Atg1T226E that was indicative of Atg1 kinase activity in vivo was still present in both atg13Δ and atg17Δ cells (Figure 10D). Therefore, the presence of a phosphomimetic within the Atg1 activation loop bypassed the normal requirement for these regulators for Atg1 autophosphorylation.
The autophagy pathway has been implicated in a wide variety of phenomena important for human health and has been suggested as a potential therapeutic target for a number of disease conditions (Levine 2005; Rubinsztein et al. 2007; Levine and Kroemer 2008). To manipulate autophagy in a productive way, it is critical that we develop a thorough understanding of how this pathway is regulated in vivo. Toward this end, the work here identifies a specific phosphorylation event within the activation loop of Atg1 as a potential point of control for autophagy in S. cerevisiae. Several observations point to the importance of this phosphorylation at position T226 for the autophagy process. First, the substitution of a nonphosphorylatable alanine residue for the threonine normally present resulted in a loss of Atg1 kinase activity and a failure to induce the autophagy process. Second, a phosphospecific antibody, prepared against a phosphopeptide corresponding to the Atg1 activation loop, recognized the wild-type Atg1 but not the Atg1T226A variant. Third, the relative level of T226 phosphorylation was found to increase specifically upon exposure to conditions that induce the autophagy process. Fourth, this antibody specifically recognized an autophosphorylated form of Atg1 that was present in cells undergoing autophagy. Fifth, we found that replacing the T226 position with an acidic residue, either glutamic or aspartic acid, resulted in an enzyme that exhibited constitutive activity with respect to autophosphorylation. This latter observation is important as it indicates that the presence of a potential phosphomimetic at position 226 in the activation loop results in the production of an active enzyme. Taken altogether, these data support the contention that Atg1 is phosphorylated within its activation loop and that this phosphorylation is important for the normal induction of the autophagy process. Since this site has been evolutionarily conserved in Atg1 orthologs, it is possible that this phosphorylation will be a point of control for autophagy in other eukaryotes.
Many protein kinases require phosphorylation within their activation loop for full activity and this modification can be a critical event in the activation of these enzymes (Nolen et al. 2004). This phosphorylation can be catalyzed by a second enzyme or can be the consequence of an autophosphorylation reaction (Lochhead et al. 2005; Imajo et al. 2006; Lochhead et al. 2006; Pirruccello et al. 2006; Oliver et al. 2007). For Atg1, the data here suggest that this modification is self-catalyzed. A functional Atg1 kinase domain was required for T226 phosphorylation in vivo and this modification occurred in vitro with Atg1 immunoprecipitates. This phosphorylation was also shown to require the presence of Atg13 and Atg17, two previously identified regulators of Atg1 kinase activity. Interestingly, replacing position T226 with a phosphomimetic, such as glutamic acid, resulted in an enzyme that no longer required Atg13 or Atg17 for autophosphorylation in vivo. This bypass of the requirement for these regulators may suggest that facilitating Atg1 autophosphorylation within the activation loop is one of the functions of the Atg13 and Atg17 proteins during the autophagy process.
Although the data here suggest that T226 phosphorylation is required for the induction of autophagy, neither the Atg1T226E nor the Atg1T226D variant was capable of supporting this degradative process in vivo. The underlying basis for this defect is not yet known, but there are a number of potential explanations that can be considered. On one extreme is the possibility that it is the threonine itself at position 226, and not its phosphorylation, that is important for Atg1 function. We do not favor this possibility given the strong correlation between T226 phosphorylation and both the induction of autophagy and the presence of Atg1 kinase activity, as measured by autophosphorylation. In addition, this model is difficult to reconcile with the observation that the presence of a phosphomimetic at position 226 led to constitutive Atg1 autophosphorylation. Therefore, we feel that the results obtained here with the Atg1T226E and Atg1T226D variants require a different explanation. One possibility is that the phosphomimetic does not fully restore the functionality of a phosphorylated threonine and therefore results in an enzyme with only partial activity. This could explain why the Atg1T226E protein is competent for autophosphorylation but not the phosphorylation of an exogenous substrate, like MBP. Alternatively, this failure to phosphorylate MBP might reflect some inherent aspect of Atg1 biology. For example, Atg1 might normally participate in a negative feedback loop that is permanently engaged in the Atg1T226E variant. Another possibility is that the autophagy defects might be due to the inability of these variants to exist in a kinase-inactive form. Previous work has indicated that Atg1 has both kinase-dependent and kinase-independent activities in the autophagy process (Abeliovich et al. 2003; Cheong et al. 2008; Kawamata et al. 2008). Atg1 may therefore need to oscillate between kinase-active and kinase-inactive states to stimulate autophagy. Clearly, further work will be necessary to distinguish between these and other potential explanations. However, we feel that the work here in its totality identifies the autophosphorylation of Atg1 within its activation loop as an important event in the normal induction of autophagy. Since Atg1 and its regulators have been conserved through evolution, a continued analysis of this modification is likely to provide additional insights into the general control of this degradative process.
We thank Daniel Klionsky, Yoshiaki Kamada, Takeshi Noda, and Yoshinori Ohsumi for reagents used in this study, Yoshiaki Kamada for helpful discussions, and Venkat Gopalan, Mark Parthun, and members of the Herman lab for comments on the manuscript. This work was supported by a grant from the National Institutes of Health (GM65227) to P.K.H.
Supporting information available at http://www.genetics.org/cgi/content/full/genetics.110.116566/DC1.
Communicating editor O. Cohen-Fix
- Received March 10, 2010.
- Accepted April 26, 2010.
- Copyright © 2010 by the Genetics Society of America