An article by R. Tamuli and D. P. Kasbekar (Genetics 178: 1169–1176) entitled “Dominant Suppression of Repeat-Induced Point Mutation in Neurospora crassa by a Variant Catalytic Subunit of DNA Polymerase-ζ” attributed the dominant repeat-induced point mutation (RIP) suppressor phenotype of the wild-isolated Adiopodoumé strain of Neurospora crassa to its allele of the upr-1 gene (upr-1Ad). The experiment upon which this attribution was based was repeated with fresh upr-1 gene disruptants, but the previous results could not be reproduced (Srividhya V. Iyer, Mukund Ramakrishnan, B. Kranthi Raj, and Durgadas P. Kasbekar, unpublished results). This invalidates the major claim of the article that the dominant RIP suppressor phenotype of the Adiopodoumé strain is due to its upr-1 allele. However, it does not invalidate the other claims made in the article: namely, (1) the existence of Srp, the dominant RIP suppressor of the Adiopodoumé strain; (2) the linkage of Srp to mat; (3) the incomplete penetrance and variable expressivity of the Srp phenotype, and (4) the diffusible nature of the RIP suppressor encoded by Srp.
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