Segregation Models for Disomic, Tetrasomic and Intermediate Inheritance in Tetraploids: A General Procedure Applied to Rorippa (Yellow Cress) Microsatellite Data
- Experimental Plant Systematics, Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, 1090 GB Amsterdam, The Netherlands
- 1↵Corresponding author: Division of Environmental and Evolutionary Biology, Graham Kerr Bldg., University of Glasgow, Glasgow, Scotland G12 8QQ. E-mail: m.stift{at}bio.gla.ac.uk
Abstract
Tetraploid inheritance has two extremes: disomic in allotetraploids and tetrasomic in autotetraploids. The possibility of mixed, or intermediate, inheritance models has generally been neglected. These could well apply to newly formed hybrids or to diploidizing (auto)tetraploids. We present a simple likelihood-based approach that is able to incorporate disomic, tetrasomic, and intermediate inheritance models and estimates the double-reduction rate. Our model shows that inheritance of microsatellite markers in natural tetraploids of Rorippa amphibia and R. sylvestris is tetrasomic, confirming their autotetraploid origin. However, in F1 hybrids inheritance was intermediate to disomic and tetrasomic inheritance. Apparently, in meiosis, chromosomes paired preferentially with the homolog from the same parental species, but not strictly so. Detected double-reduction rates were low. We tested the general applicability of our model, using published segregation data. In two cases, an intermediate inheritance model gave a better fit to the data than the tetrasomic model advocated by the authors. The existence of inheritance intermediate to disomic and tetrasomic has important implications for linkage mapping and population genetics and hence breeding programs of tetraploids. Methods that have been developed for either disomic or tetrasomic tetraploids may not be generally applicable, particularly in systems where hybridization is common.
Footnotes
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↵2 Present address: ETH Zürich, Experimental Ecology, Institute of Integrative Biology, CHN J14, 8092, Zürich, Switzerland.
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Communicating editor: O. Savolainen
- Received December 22, 2007.
- Accepted May 25, 2008.
- Copyright © 2008 by the Genetics Society of America
