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Lawrence N Yager, Hyung-Ok Lee, Deborah L Nagle, John E Zimmerman, Analysis of fluG Mutations That Affect Light-Dependent Conidiation in Aspergillus nidulans, Genetics, Volume 149, Issue 4, 1 August 1998, Pages 1777–1786, https://doi.org/10.1093/genetics/149.4.1777
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Abstract
Conidiation in Aspergillus nidulans is induced by exposure to red light but can also be induced by blue light in certain mutant strains. We have isolated a mutation in the fluG gene that abolishes responsiveness to red light but does not affect the response to blue light. It has been shown that the veA1 (velvet) mutation allows conidiation to occur in the absence of light. We have identified three other fluG mutations that suppress the veA1 phenotype; these double mutants do not conidiate in the dark. The mutations described here define two new phenotypic classes of fluG alleles that display abnormal responses to light. We have characterized these mutations with respect to their molecular identity and to their effect on fluG transcription. Although it has been shown that fluG is required for the synthesis of an extracellular factor that directs conidiation, we do not detect this factor under conditions that promote conidiation in the veA1 suppressors. Furthermore, extracellular rescue is not observed in fluG deletion strains containing the wild-type veA allele. We propose that a genetic interaction between fluG and veA influences the production of the extracellular signal and regulates the initiation of conidiation.
CONIDIATION in the filamentous ascomycete Aspergillus nidulans involves the elaboration of haploid, uninucleate spores on a complex multicellular apparatus, called a conidiophore, that consists of a stalk, vesicle and sterigmata (metulae and phialides). Although the production of conidia results from the coordinate expression of several hundred genes, only three genes, brlA, abaA, and wetA, are required for the transition from vegetative growth to asexual sporulation (Martinelli and Clutterbuck 1971; Timberlake 1980; Boylan et al. 1987; Adams et al. 1988; Mirabito et al. 1989; Marshall and Timberlake 1991). These three genes form a sequentially expressed pathway, the activation of which is dependent upon at least six genes, designated fluG, flbA, flbB, flbC, flbD, and flbE. These latter genes were identified by mutations resulting in the proliferation of undifferentiated masses of vegetative hyphae that give colonies a “fluffy” appearance (Adams et al. 1992; Lee and Adams 1994a,b; Wieser et al. 1994; Wieser and Adams 1995). One of these genes, fluG, has been cloned and shown to encode an 864-amino acid polypeptide of unknown function that shares limited identity with type I prokaryotic glutamine synthetases (GSI) (Lee and Adams 1994a). It has been proposed that fluG is responsible for the synthesis of a diffusible, low molecular weight factor that controls the initiation of sporulation (Lee and Adams 1994a). Evidence for this was obtained by demonstrating that the fluG mutant phenotype could be suppressed by growing the mutant strain next to a wild-type strain, producing a strong band of conidiation between the two colonies. Furthermore, the phenotypic rescue of the fluG mutant strain occurred even if the two colonies were separated by dialysis membrane having a pore size of 6–8 kD.
The onset of conidiation is complex and controlled by both genetically determined factors and environmental conditions. It has been previously shown that conidiation is normally inhibited in submerged culture but readily takes place when mycelia are exposed to an air interface (Axelrod 1972; Axelrod et al. 1973). An important but often neglected environmental condition that also influences conidiation is light (Mooney and Yager 1990). Light dependence is determined by the allelic state of the veA (velvet) gene. Wild-type strains conidiate in the light but are aconidial in the dark, whereas strains bearing the veA1 mutation conidiate in the absence of light.
Conidiation is elicited by exposure to red light in the range 690–710 nm (Mooney and Yager 1990). Champe et al. (1994) showed that light also affects sexual sporulation, causing a delay in the formation of cleistothecial foci, and that this delay is elicited by the same range of wavelengths that induce conidiation. In addition, these researchers identified a mutation, designated bliA1 (blue light inducible; formally edbA1), that causes delayed cleistotheciation if colonies are exposed to blue light at 436 nm. We have shown that conidiation is also elicited by blue light in strains carrying the bliA1 mutation (L. N. Yager, unpublished results).
To help elucidate how light influences development, we have isolated and characterized a mutation in the fluG gene that abolishes sensitivity to red light but maintains the ability to respond to blue light. In addition, three extragenic suppressors of the veA1 mutation (Mooney et al. 1990) are shown to be alleles of fluG. The data presented indicate that fluG and veA are involved in the interpretation of the light signal and suggest a genetic interaction between these two genes.
MATERIALS AND METHODS
Aspergillus strains, genetic techniques and growth conditions: The strains used are listed in Table 1. Genotypes are designated as in Clutterbuck (1984). Standard genetic techniques were used throughout this study (Pontecorvo et al. 1953; Clutterbuck 1977; Käfer 1977).
The complete growth medium and general culture techniques that were employed are described in Yager et al. (1982) and Butnick et al. (1984). Incubations were performed either at 32° or 42°. Surface-grown colonies requiring illumination with white light were incubated in incubators (model I-35LL or model I-30BL; Percival Mfg. Co., Boone, IA) equipped with General Electric 20-W broad-spectrum fluorescent light bulbs positioned at a distance of 20 cm from the agar surface (average fluence rate was 35 μE m−2 sec−1). Dark conditions were obtained by loosely wrapping single plates in aluminum foil. Broad wavelength red light (610 to 720 nm) was produced either by wrapping General Electric 20-W broad-spectrum fluorescent light bulbs housed in the Percival incubator with GAMCOLOR sharp cutoff gelatin filters or by placing a sheet of Red Shinkolite (Argo Plastic, Los Angeles, CA) between the bulbs and petri plates. In both cases, the average fluence rate was 20 μE m−2 sec−1. All fluence rates were measured with a photometer (Li-Corp. Inc., Lincoln, NE), model LI-189.
Strains of Aspergillus nidulans that are responsive to blue light display a sharp peak of sensitivity at 436 nm (Champe et al. 1994; L. N. Yager, unpublished results). Blue light was produced by passing white light from either a projector lamp bulb (DFD, 120 V, 1000 W; Philips Lighting Co., Somerset, NJ) or an Osram tungsten-halogen incandescent lamp (FA4A, 120 V, 500 W) through a Corion (Holliston, MA) 436 ± 10-nm interference filter. Heat from the lamp was dissipated by directing forced air from a Shaded-Pole blower (model no. 4C441A; Dayton) onto the lamp. Heat was further reduced by passing the light generated from the lamp through a glass vessel (10 × 6 × 15 mm) containing 1.5 mm CuSO4· 5 H2O and focusing the light through a lens (U.S. Precision Lens, Inc.) onto the interference filter. The interference filter was fitted in a mask of black poster board cut to cover the lid of the petri dish. A piece of the same black poster board was also placed on the bottom of the dish, and the edges were wrapped with aluminum foil during incubation to reduce light scattering. The intensity of focused light was controlled by connecting the light source to a variable autotransformer (Powerstat model 1126 or 3PN16B). The average fluence rate of blue light produced by this apparatus was 5 μE m−2 sec−1.
Colonies do not normally conidiate in submerged culture but do so readily after they have acquired developmental competence and are exposed to air (Axelrod et al. 1973). The transfer of mycelia from liquid to solid culture, termed induction, is used to initiate and synchronize conidiation (Timberlake 1980). For conidial yield determinations and RNA analysis, colonies were induced at 36 hr after inoculation of spores into liquid medium, which is well after the acquisition of competence, exposed to the appropriate illumination condition and then harvested at the designated time. Conidial yield determinations were performed as described in Yager et al. (1982). Radial colonial growth rates, which obey a linear growth relationship, were determined as described in Yager et al. (1982). The time of conidiophore vesicle appearance was scored by microscopic examination using the method of Axelrod et al. (1973).
Isolation of a red light-insensitive mutant: Approximately 5 × 104 conidia of strain WIM 126 (yA2 pabaA1; veA+ (bliA1)) were suspended in 10 ml of deionized water and irradiated with ultraviolet light at a dosage that produced 10% survival (700 μW cm−2 for 40 sec). A total of 5 × 105 conidia were mutagenized, plated at a density of 100 conidia/plate, and incubated in broad spectrum red light for 3 days at 32°. Approximately 500 presumptive aconidial colonies were selected and screened for their responses to white and blue light. Two mutants that failed to conidiate in red light, but conidiated in white and blue light, were isolated. However, only one mutant was selected for study; the other displayed a leaky aconidial phenotype that interfered with analysis. The selected isolate was backcrossed to TU 95 (nicB8; veA+ (bliA1)) before further analysis.
Nucleic acid manipulations: A. nidulans DNA-mediated transformation was performed as described by Yelton et al. (1984) and Miller et al. (1985). Standard methods for construction, maintenance, and isolation of recombinant plasmids were used (Sambrook et al. 1989). All wild-type fluG genomic DNA fragments were obtained from plasmid pFM1 (Adams et al. 1992), which contains the entire fluG gene within a 7-kb BamHI-HindIII fragment. Fragments tested for mutant complementation following transformation were subcloned into pPK1 or pSM3. Plasmid pPK1 was constructed by replacing the f1 origin of replication located at the SspI site in pBluescript SK− (Stratagene, La Jolla, CA) with the argB gene contained in the 1.9-kb BamHI fragment from pSALARGB (Berse et al. 1983). Plasmid pSM3 was constructed by replacing the f1 origin of replication located at the SspI site in pBluescript SK− with the pyroA gene contained in the 1.9-kb XbaI-BamHI fragment from p14 (May et al. 1989).
To isolate the fluG701 allele, a genomic library was first constructed from strain TU 114 (yA2 pabaA1; fluG701; veA1). Fragments between 6 and 9 kb were isolated from BamHI-HindIII-restricted DNA and inserted into pGEM3Zf(−) (Promega, Madison, WI). The probe used to screen this library was prepared by first isolating the 2.5-kb XhoI fragment from pFM1 and then radiolabeling the fragment with 32P by random priming. The recovered plasmid was designated pJZ1.
DNA sequencing on isolated clones was performed by the chain-termination method using the Sequenase V2.0 kit (Amersham, Clearbrook, IL) following the procedure provided by the manufacturer. Uncloned genomic DNA was sequenced using the Sequitherm Cycle Sequencing kit (Epicentre Technologies, Madison, WI) according to the manufacturer's directions. The primers used for both procedures are listed below and designated as follows (name of oligonucleotide, sequence, location of 5′ end, annealing temperature used for TAQ cycle sequencing): TA141-8, CGTCGGCTGATCAATTG, 32, 54°; TA141-3, AGGTCTCCCCATTCATC, 125, 50°; TA141-12, GCAGATCATATCTGAGG, 406, 48°; FLUG-04, ATGACGAGCTTTGGT, 513, 44°; FLUG-07, TTTGGGAGTCGTTCT, 789, 42°; BN1, CAGCAGATTGATGTC,1115, 42°; ACODFUS1, GCCAGATATCCAGCCTC, 1222, 54°; PEP-8, CTGCATTCTGCTGAT, 1424, 42°; PEP-4, TCAATGGTATAATCACC, 1507, 44°; FLUG-15, TGGTGCCCAGACGAT, 1609, 46°; FLUG-18, TTATACCAGACCTAT, 1851, 38°; PEP-7, CCAAACTCGTCTTTGAG, 2008, 48°; PEP-1, ACCCATCAACAGGAGAGG, 2064, 54°; PEP-6, TTGTCAGGTGGGAGGATG, 2260, 54°; FLUG-23, TTCCATCTCCCCTTCAA, 2395, 46°; PEP-5, TTAACGCGGTCGTAGC, 2506, 48°; FLUG-26, CTCACTATCAAGGATT, 2687, 42°; BN3, GATTAGACTCAATACCTC, 3000, 46°; and FLUG-30, AGGCCGTGGAAATATT, 3036, 44°. The location of each oligonucleotide refers to its position relative to the sequence of the fluG gene shown in Lee and Adams (1994a). FLUG oligonucleotides were derived in this laboratory. TA, BN, PEP, and ACODFUS1 oligonucleotides were donated by Thomas Adams. Any differences observed between mutant and wild-type sequences for those results obtained from uncloned DNA were verified by repeating the sequencing from three independently isolated batches of DNA. Additional confirmation was obtained by sequencing both strands of DNA around the location of each mutation.
Total RNA was isolated as described in Mooney and Yager (1990), separated by electrophoresis in formaldehyde-agarose gels, and transferred without pretreatment to nylon membranes (Hybond-N; Amersham, Clearbrook, IL). RNA was bound to membranes and hybridized to 32P-labeled random primed probes according to the procedures recommended by the manufacturer. The 2.5-kb XhoI fragment from pFM1 was used as a fluG-specific probe. The 1.9-kb BamHI fragment from pSALARGB was used as an argB-specific probe. Both fragments were gel purified before labeling.
RESULTS
Isolation and genetic analysis of a mutant that fails to conidiate in red light: Mutagenesis of the veA+; bliA1 strain, WIM 126 (Table 1), yielded a red light-insensitive mutant that was identified by its ability to produce normal developmental structures in either blue light or full spectrum white light, but not in red light. To demonstrate that this mutation behaved as a single Mendelian allele, the mutant strain was meiotically crossed to TU
Designation . | Genotype . | Source . |
---|---|---|
FGSC A4 | veA+ (bliA+) | Fungal Genetics Stock Center (FGSC) |
FGSC A283 | adE20 suA1adE20yA2; AcrA1; galA1; pyroA4; facA303; sB3;nicB8; riboB2 veA1 | FGSC |
FGSC A457 | proA1 biA1; galE9 sc12 dilA1 phenA2; choAl | FGSC |
FGSC A503 | proA1 yA2; methH2 dilA1 | FGSC |
M3188 | biAl; argB2; methG1; veA1 | G. May |
THL135.1 | yA2pabaA1; ΔfluG::argB argB2; pyroA4; veA+ | This study |
TTA127.4 | yA2pabaA1; ΔfluG::trpC; trpC801 veA1 | Lee and Adams (1994a) |
TU1 | pyroA4; veA1 | This lab |
TU5 | fluG102 pyroA4; veA1 | This lab |
TU11 | fluG684; pyroA4; veA1 | This lab |
TU 39a | fluG10sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 41b | fluG20sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 42c | fluG30sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 49c | yA2pabaA1; fluG30sveA1; veA1 | Mooney et al. (1990) |
TU 53a | yA2pabaA1; fluG10sveA1; veA1 | Mooney et al. (1990) |
TU 55b | yA2pabaA1; fluG20sveA1; veA1 | Mooney et al. (1990) |
TU 91 | yA2pabaA1; fluG701; veA+ (bliA1) | This study |
TU 95 | nicB8; veA+ (bliA1) | This lab |
TU 110 | pabaA1; fluG701; veA+ (bliA1) | This study |
TU 108 | pabaA1; fluG701; veA1 | This study |
TU 114 | yA2pabaA1; fluG701; veA1 | This study |
TU 120 | fluG701; wA3 pyroA4; veA1 | This study |
TU 135 | ya2pabaA1; argB2; pyroA4; veA+ | This lab |
TU 136 | yA2pabaA1; fluG701 argB2; veA1 | This study |
WIM 009 | fluG102 veA+ | Yager et al. (1982) |
WIM 020 | fluG684; veA+ | Yager et al. (1982) |
WIM 064 | yA2pabaA1; veA1 | S. P. Champe |
WIM 126 | yA2 pabaA1; veA+ (bliA1) | S. P. Champe |
Designation . | Genotype . | Source . |
---|---|---|
FGSC A4 | veA+ (bliA+) | Fungal Genetics Stock Center (FGSC) |
FGSC A283 | adE20 suA1adE20yA2; AcrA1; galA1; pyroA4; facA303; sB3;nicB8; riboB2 veA1 | FGSC |
FGSC A457 | proA1 biA1; galE9 sc12 dilA1 phenA2; choAl | FGSC |
FGSC A503 | proA1 yA2; methH2 dilA1 | FGSC |
M3188 | biAl; argB2; methG1; veA1 | G. May |
THL135.1 | yA2pabaA1; ΔfluG::argB argB2; pyroA4; veA+ | This study |
TTA127.4 | yA2pabaA1; ΔfluG::trpC; trpC801 veA1 | Lee and Adams (1994a) |
TU1 | pyroA4; veA1 | This lab |
TU5 | fluG102 pyroA4; veA1 | This lab |
TU11 | fluG684; pyroA4; veA1 | This lab |
TU 39a | fluG10sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 41b | fluG20sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 42c | fluG30sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 49c | yA2pabaA1; fluG30sveA1; veA1 | Mooney et al. (1990) |
TU 53a | yA2pabaA1; fluG10sveA1; veA1 | Mooney et al. (1990) |
TU 55b | yA2pabaA1; fluG20sveA1; veA1 | Mooney et al. (1990) |
TU 91 | yA2pabaA1; fluG701; veA+ (bliA1) | This study |
TU 95 | nicB8; veA+ (bliA1) | This lab |
TU 110 | pabaA1; fluG701; veA+ (bliA1) | This study |
TU 108 | pabaA1; fluG701; veA1 | This study |
TU 114 | yA2pabaA1; fluG701; veA1 | This study |
TU 120 | fluG701; wA3 pyroA4; veA1 | This study |
TU 135 | ya2pabaA1; argB2; pyroA4; veA+ | This lab |
TU 136 | yA2pabaA1; fluG701 argB2; veA1 | This study |
WIM 009 | fluG102 veA+ | Yager et al. (1982) |
WIM 020 | fluG684; veA+ | Yager et al. (1982) |
WIM 064 | yA2pabaA1; veA1 | S. P. Champe |
WIM 126 | yA2 pabaA1; veA+ (bliA1) | S. P. Champe |
TU and THL strains were derived in this laboratory, FGSC strains were obtained from the Fungal Genetics Stock Center, the M strain was obtained from Greg May, the TTA strain was obtained from Thomas Adams, and the WIM strains were obtained from Sewell P. Champe. Strains bearing the bliA1 allele conidiate in response to blue light. This allele is indicated in parentheses because it has not been assigned to a specific locus. Only strains tested for blue light responsiveness were designated bliA1 or bliA+.
The suClveA1 mutation has been renamed fluG10sveA1. TU 53 is available from the FGSC as strain A861.
The suC2veA1 mutation has been renamed fluG20sveA1. TU 55 is available from the FGSC as strain A863.
The suC3veA1 mutation has been renamed fluG30sveA1. TU 49 is available from the FGSC as strain A858.
Designation . | Genotype . | Source . |
---|---|---|
FGSC A4 | veA+ (bliA+) | Fungal Genetics Stock Center (FGSC) |
FGSC A283 | adE20 suA1adE20yA2; AcrA1; galA1; pyroA4; facA303; sB3;nicB8; riboB2 veA1 | FGSC |
FGSC A457 | proA1 biA1; galE9 sc12 dilA1 phenA2; choAl | FGSC |
FGSC A503 | proA1 yA2; methH2 dilA1 | FGSC |
M3188 | biAl; argB2; methG1; veA1 | G. May |
THL135.1 | yA2pabaA1; ΔfluG::argB argB2; pyroA4; veA+ | This study |
TTA127.4 | yA2pabaA1; ΔfluG::trpC; trpC801 veA1 | Lee and Adams (1994a) |
TU1 | pyroA4; veA1 | This lab |
TU5 | fluG102 pyroA4; veA1 | This lab |
TU11 | fluG684; pyroA4; veA1 | This lab |
TU 39a | fluG10sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 41b | fluG20sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 42c | fluG30sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 49c | yA2pabaA1; fluG30sveA1; veA1 | Mooney et al. (1990) |
TU 53a | yA2pabaA1; fluG10sveA1; veA1 | Mooney et al. (1990) |
TU 55b | yA2pabaA1; fluG20sveA1; veA1 | Mooney et al. (1990) |
TU 91 | yA2pabaA1; fluG701; veA+ (bliA1) | This study |
TU 95 | nicB8; veA+ (bliA1) | This lab |
TU 110 | pabaA1; fluG701; veA+ (bliA1) | This study |
TU 108 | pabaA1; fluG701; veA1 | This study |
TU 114 | yA2pabaA1; fluG701; veA1 | This study |
TU 120 | fluG701; wA3 pyroA4; veA1 | This study |
TU 135 | ya2pabaA1; argB2; pyroA4; veA+ | This lab |
TU 136 | yA2pabaA1; fluG701 argB2; veA1 | This study |
WIM 009 | fluG102 veA+ | Yager et al. (1982) |
WIM 020 | fluG684; veA+ | Yager et al. (1982) |
WIM 064 | yA2pabaA1; veA1 | S. P. Champe |
WIM 126 | yA2 pabaA1; veA+ (bliA1) | S. P. Champe |
Designation . | Genotype . | Source . |
---|---|---|
FGSC A4 | veA+ (bliA+) | Fungal Genetics Stock Center (FGSC) |
FGSC A283 | adE20 suA1adE20yA2; AcrA1; galA1; pyroA4; facA303; sB3;nicB8; riboB2 veA1 | FGSC |
FGSC A457 | proA1 biA1; galE9 sc12 dilA1 phenA2; choAl | FGSC |
FGSC A503 | proA1 yA2; methH2 dilA1 | FGSC |
M3188 | biAl; argB2; methG1; veA1 | G. May |
THL135.1 | yA2pabaA1; ΔfluG::argB argB2; pyroA4; veA+ | This study |
TTA127.4 | yA2pabaA1; ΔfluG::trpC; trpC801 veA1 | Lee and Adams (1994a) |
TU1 | pyroA4; veA1 | This lab |
TU5 | fluG102 pyroA4; veA1 | This lab |
TU11 | fluG684; pyroA4; veA1 | This lab |
TU 39a | fluG10sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 41b | fluG20sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 42c | fluG30sveA1; pyroA4; veA1 | Mooney et al. (1990) |
TU 49c | yA2pabaA1; fluG30sveA1; veA1 | Mooney et al. (1990) |
TU 53a | yA2pabaA1; fluG10sveA1; veA1 | Mooney et al. (1990) |
TU 55b | yA2pabaA1; fluG20sveA1; veA1 | Mooney et al. (1990) |
TU 91 | yA2pabaA1; fluG701; veA+ (bliA1) | This study |
TU 95 | nicB8; veA+ (bliA1) | This lab |
TU 110 | pabaA1; fluG701; veA+ (bliA1) | This study |
TU 108 | pabaA1; fluG701; veA1 | This study |
TU 114 | yA2pabaA1; fluG701; veA1 | This study |
TU 120 | fluG701; wA3 pyroA4; veA1 | This study |
TU 135 | ya2pabaA1; argB2; pyroA4; veA+ | This lab |
TU 136 | yA2pabaA1; fluG701 argB2; veA1 | This study |
WIM 009 | fluG102 veA+ | Yager et al. (1982) |
WIM 020 | fluG684; veA+ | Yager et al. (1982) |
WIM 064 | yA2pabaA1; veA1 | S. P. Champe |
WIM 126 | yA2 pabaA1; veA+ (bliA1) | S. P. Champe |
TU and THL strains were derived in this laboratory, FGSC strains were obtained from the Fungal Genetics Stock Center, the M strain was obtained from Greg May, the TTA strain was obtained from Thomas Adams, and the WIM strains were obtained from Sewell P. Champe. Strains bearing the bliA1 allele conidiate in response to blue light. This allele is indicated in parentheses because it has not been assigned to a specific locus. Only strains tested for blue light responsiveness were designated bliA1 or bliA+.
The suClveA1 mutation has been renamed fluG10sveA1. TU 53 is available from the FGSC as strain A861.
The suC2veA1 mutation has been renamed fluG20sveA1. TU 55 is available from the FGSC as strain A863.
The suC3veA1 mutation has been renamed fluG30sveA1. TU 49 is available from the FGSC as strain A858.
95. Analysis of 120 progeny showed that the mutation segregated from its respective wild-type allele in a 1:1 ratio (wild-type:mutant; 59:61; χ2 = 0.034, d.f. = 1, P > 0.9) and assorted independently from unlinked mutations (P > 0.99 for nicB8, yA2, and pabaA1; χ2 test).
The red light-insensitive mutant displays an aconidial phenotype at 42° regardless of illumination in addition to its inability to respond to red light at 32°. Unlike the red light-insensitive phenotype, the temperature-sensitive aconidial phenotype is observed in strains carrying either veA+ or veA1 alleles. Both the red light-insensitive and temperature-sensitive aconidial phenotypes were found to cosegregate in an analysis of the progeny from the above cross and other crosses not shown. Although the genetic analyses described below were simplified by assaying for the temperature-sensitive phenotype, selected progeny from each cross were also examined for the red light-insensitive phenotype. In all cases, both phenotypes cosegregated and resulted in identical conclusions.
The red light-insensitive mutation was tested for dominance by constructing a heterozygous diploid. Table 2 shows that this diploid was conidial and indistinguishable from a homozygous wild-type control, indicating that the red light-insensitive mutation (designated fluG701 for reasons described below) is recessive to its wild-type allele. Diploids heterozygous for the red light-insensitive mutation and carrying multiple genetic markers were next constructed and subjected to mitotic haploidization. Analysis of the resulting progeny indicated that the red light-insensitive mutation is linked to chromosome III. Furthermore, analysis of progeny from meiotic crosses to chromosome III markers positioned the red light-insensitive mutation within 0.4% recombination distance from the fluG102 mutation (4 wild-type recombinants recovered from 2000 total progeny) and <0.1% recombination distance from the fluG684 mutation (0 wild-type recombinants recovered from 2000 total progeny). These latter two mutations display a
Genotype . | Phenotype . |
---|---|
+/fluG701 | Conidial |
fluG701/fluG701 | Fluffy aconidial |
+/+ | Conidial |
fluG701/fluG102 | Fluffy aconidial |
fluG701/fluG684 | Fluffy aconidial |
fluG10sveA1/fluG102 | Fluffy aconidial |
fluG10sveA1/fluG684 | Fluffy aconidial |
fluG10sveA1/fluG10sveA1 | Fluffy aconidial |
+/fluG10sveA1 | Conidial |
Genotype . | Phenotype . |
---|---|
+/fluG701 | Conidial |
fluG701/fluG701 | Fluffy aconidial |
+/+ | Conidial |
fluG701/fluG102 | Fluffy aconidial |
fluG701/fluG684 | Fluffy aconidial |
fluG10sveA1/fluG102 | Fluffy aconidial |
fluG10sveA1/fluG684 | Fluffy aconidial |
fluG10sveA1/fluG10sveA1 | Fluffy aconidial |
+/fluG10sveA1 | Conidial |
Colonies were incubated at 42° in continuous white light at an intensity of 35 μE m−2 sec−1 and then scored at 48 hr. All diploids were constructed in a homozygous veA1 background.
Genotype . | Phenotype . |
---|---|
+/fluG701 | Conidial |
fluG701/fluG701 | Fluffy aconidial |
+/+ | Conidial |
fluG701/fluG102 | Fluffy aconidial |
fluG701/fluG684 | Fluffy aconidial |
fluG10sveA1/fluG102 | Fluffy aconidial |
fluG10sveA1/fluG684 | Fluffy aconidial |
fluG10sveA1/fluG10sveA1 | Fluffy aconidial |
+/fluG10sveA1 | Conidial |
Genotype . | Phenotype . |
---|---|
+/fluG701 | Conidial |
fluG701/fluG701 | Fluffy aconidial |
+/+ | Conidial |
fluG701/fluG102 | Fluffy aconidial |
fluG701/fluG684 | Fluffy aconidial |
fluG10sveA1/fluG102 | Fluffy aconidial |
fluG10sveA1/fluG684 | Fluffy aconidial |
fluG10sveA1/fluG10sveA1 | Fluffy aconidial |
+/fluG10sveA1 | Conidial |
Colonies were incubated at 42° in continuous white light at an intensity of 35 μE m−2 sec−1 and then scored at 48 hr. All diploids were constructed in a homozygous veA1 background.
temperature-sensitive aconidial phenotype with the concomitant production of fluffy, aerial hyphae (Yager et al. 1982; Adams et al. 1992). To show whether the red light-insensitive mutation is an allele of fluG, heterozygous diploids were constructed between the mutation and either fluG102 or fluG684. Table 2 shows that both diploids display the fluffy, temperature-sensitive aconidial phenotype characterized by the homozygous diploid control and parental mutant strains. These data indicate that the red light-insensitive mutation is an allele of fluG and is thus designated fluG701.
Characterization of development and vegetative growth: Figure 1A shows that there is no significant difference in conidial yield between fluG701 and wild-type strains for colonies incubated either in full spectrum white light or in blue light. However, incubation in red light decreases the conidial yield of the mutant strain by a factor of approximately 105 conidia per colony from that observed in the wild-type strain, a level equivalent to dark levels. As expected, conidial yields are negligible in all dark-grown colonies.
To show whether the fluG701 mutation also affects vegetative growth, radial growth-rate measurements were performed on wild-type and mutant strains under different illumination conditions. The vegetative growth rate is not significantly affected by different illumination conditions or by the allelic state of fluG (Figure 1B). Furthermore, fluG701 does not affect developmental kinetics. The time of conidiophore vesicle appearance under those conditions that promote conidiation is not significantly different between mutant and wild-type strains (6 ± 0.5 hr).
Suppressors of the veA1 mutation are alleles of fluG: Mooney et al. (1990) identified six extragenic suppressors of the veA1 mutation that restore light-dependent conidiation. Three of these suppressors, suC1veA1, suC2-veA1, and suC3veA1, define a single complementation group that is linked to chromosome III and whose veA1 suppressors also display a temperature-sensitive aconidial, fluffy phenotype. We have previously shown that this temperature-sensitive phenotype cosegregates with restoration of the light-dependent conidiation phenotype (Mooney et al. 1990) and have used this temperature-sensitive characteristic to simplify the genetic analysis of these mutations. Construction of heterozygous diploids between suC1veA1 and either fluG102 or fluG684 yield colonies displaying a temperature-sensitive aconidial, fluffy phenotype that is indistinguishable from homozygous diploid and parental strain controls (Table 2). Since these data indicate that suC1veA1 is an allele of fluG, this mutation has been renamed fluG10sveA1. Similarly, suC2veA1 will be referred to as fluG20sveA1, and suC3veA1 will be referred to as fluG30sveA1.
Table 3 classifies the known fluG alleles into five groups according to their conidial response to different illumination and temperature conditions. A comparison of the effect of the allelic state of the veA gene on
the phenotype of the different fluG alleles is also shown. The first class, defined by the single member fluG701, contains the only mutation that fails to conidiate in red light at 32°. Placing this mutation into a veA1 background allows conidiation to occur both in the dark and in red light. The second class contains mutations of fluG that suppress the veA1 phenotype. These mutations restore light-dependent conidiation in strains bearing the veA1 mutation. Placing these mutations into a veA+ background does not affect the conidial phenotype. The third class contains mutations that display a temperature-sensitive aconidial phenotype but do not show an abnormal response to light at the permissive temperature. Their conidial phenotype at 32° is identical to the wild type in both veA+ and veA1 backgrounds. The fourth class is defined by a fluG deletion mutation that is aconidial in all illumination and temperature conditions and whose conidial phenotype is unaffected by the allelic state of the veA gene. These four classes of fluG mutations all show an aconidial phenotype at 42°. The fifth class describes the phenotype of the wild-type fluG allele. Although fluG+; veA+ strains display light-dependent conidiation at 32°, conidiation occurs at 42° regardless of the presence or absence of light.
Although the allelic state of veA determines the conidial response to light, it also affects colony morphology. Table 3 shows that all fluG mutant strains that contain the veA1 allele produce fluffy, aerial hyphae under conditions that suppress conidiation, a common characteristic of all previously described fluG mutations (Yager et al. 1982; Adams et al. 1992). However, fluffy, aerial hyphae are not observed under conditions that result in an aconidial phenotype either in fluG701 or the veA1 suppressor strains that contain the veA+ allele.
Molecular identification of fluG mutations: Transformation events in A. nidulans can result in ectopic integration of exogenous DNA into the genome (Yelton et al. 1984). Nevertheless, a mutation can be “repaired” by a recombinational event that replaces the faulty region with wild-type sequences, if a homologous recombinational event can be selected at the endogenous target gene. Complementation of the mutant phenotype after transformation with different restriction enzyme fragments derived from the wild-type fluG clone, pFM1, was used to localize the various light-dependent mutations to specific regions within the fluG gene.
The fluG gene is contained within the 7-kb BamHI-HindIII restriction fragment shown in Figure 2A. This fragment complements the fluG701, fluG10sveA1, fluG20sveA1, and fluG30sveA1 mutations. Both fluG701 and fluG10sveA1 are contained within the 1.9-kb XhoI-EcoRI fragment as indicated by complementation with the 4.3-kb BamHI-EcoRI and 2.8-kb internal XhoI fragments but not with the 2.7-kb EcoRI-HindIII fragment. Similarly, the fluG30sveA1 mutation is contained within the 0.9-kb EcoRI-XhoI fragment as demonstrated by complementation with the 2.8-kb internal XhoI and 2.7-kb EcoRI-HindIII fragments. The fluG20sveA1 mutation is located within the 2.8-kb internal XhoI fragment as indicated by complementation only by this subfragment from the entire 7-kb BamHI-HindIII restriction fragment.
The molecular identity of each fluG light-dependent mutation was determined by DNA sequencing of the smallest overlapping region among restriction fragments that complement each mutant phenotype. In the case of fluG701, the BamHI-HindIII fragment containing the mutant sequences was first cloned from strain TU 114 (Table 1) and the 2.8-kb internal XhoI fragment was later sequenced. As shown in Figure 2B, the fluG701
. | . | . | Phenotypes . | ||||||
---|---|---|---|---|---|---|---|---|---|
. | . | . | 32° . | 42° . | |||||
Class . | Allele . | Genotype . | WL . | RL . | BL . | D . | WL . | RL . | D . |
Red light insensitive | fluG701 | fluG701; veA+ | C | A | C | A | A | A | A |
fluG701; veA1 | C | C | C | C | FA | FA | FA | ||
veA1 suppressor | fluG10sveA1 | fluG10sveA1; veA+ | C | C | ND | A | A | A | A |
fluG20sveA1 | fluG10sveA1; veA1 | C | C | ND | FA | FA | FA | FA | |
fluG30sveA1 | |||||||||
Temperature sensitive | fluG102 | fluG102; veA+ | C | C | ND | A | FA | FA | FA |
fluG684 | fluG102, veA1 | C | C | ND | C | FA | FA | FA | |
Null | ΔfluG | ΔfluG; veA+ | FA | FA | ND | FA | FA | FA | FA |
ΔfluG; veA1 | FA | FA | ND | FA | FA | FA | FA | ||
Wild type | fluG+ | fluG+; veA+ | C | C | C | A | C | C | C |
fluG+; veA1 | C | C | C | C | C | C | C |
. | . | . | Phenotypes . | ||||||
---|---|---|---|---|---|---|---|---|---|
. | . | . | 32° . | 42° . | |||||
Class . | Allele . | Genotype . | WL . | RL . | BL . | D . | WL . | RL . | D . |
Red light insensitive | fluG701 | fluG701; veA+ | C | A | C | A | A | A | A |
fluG701; veA1 | C | C | C | C | FA | FA | FA | ||
veA1 suppressor | fluG10sveA1 | fluG10sveA1; veA+ | C | C | ND | A | A | A | A |
fluG20sveA1 | fluG10sveA1; veA1 | C | C | ND | FA | FA | FA | FA | |
fluG30sveA1 | |||||||||
Temperature sensitive | fluG102 | fluG102; veA+ | C | C | ND | A | FA | FA | FA |
fluG684 | fluG102, veA1 | C | C | ND | C | FA | FA | FA | |
Null | ΔfluG | ΔfluG; veA+ | FA | FA | ND | FA | FA | FA | FA |
ΔfluG; veA1 | FA | FA | ND | FA | FA | FA | FA | ||
Wild type | fluG+ | fluG+; veA+ | C | C | C | A | C | C | C |
fluG+; veA1 | C | C | C | C | C | C | C |
Colonies were incubated either in white light (WL; 35 μE m−2 sec−1), red light (RL; 610 to 720 nm; 20 μE m−2 sec−1), blue light (BL; 436 nm; 5 μE m−2 sec−1), or the dark (D). All phenotypes were examined on complete medium. 42° in blue light was not done. The fluG701 and wild-type veA+ strains contain the bliA1 allele. The symbols represent the phenotype of colonies possessing the indicated allele. C, conidial; A, aconidial; FA, aconidial with fluffy, profuse aerial hyphae; ND, not done.
. | . | . | Phenotypes . | ||||||
---|---|---|---|---|---|---|---|---|---|
. | . | . | 32° . | 42° . | |||||
Class . | Allele . | Genotype . | WL . | RL . | BL . | D . | WL . | RL . | D . |
Red light insensitive | fluG701 | fluG701; veA+ | C | A | C | A | A | A | A |
fluG701; veA1 | C | C | C | C | FA | FA | FA | ||
veA1 suppressor | fluG10sveA1 | fluG10sveA1; veA+ | C | C | ND | A | A | A | A |
fluG20sveA1 | fluG10sveA1; veA1 | C | C | ND | FA | FA | FA | FA | |
fluG30sveA1 | |||||||||
Temperature sensitive | fluG102 | fluG102; veA+ | C | C | ND | A | FA | FA | FA |
fluG684 | fluG102, veA1 | C | C | ND | C | FA | FA | FA | |
Null | ΔfluG | ΔfluG; veA+ | FA | FA | ND | FA | FA | FA | FA |
ΔfluG; veA1 | FA | FA | ND | FA | FA | FA | FA | ||
Wild type | fluG+ | fluG+; veA+ | C | C | C | A | C | C | C |
fluG+; veA1 | C | C | C | C | C | C | C |
. | . | . | Phenotypes . | ||||||
---|---|---|---|---|---|---|---|---|---|
. | . | . | 32° . | 42° . | |||||
Class . | Allele . | Genotype . | WL . | RL . | BL . | D . | WL . | RL . | D . |
Red light insensitive | fluG701 | fluG701; veA+ | C | A | C | A | A | A | A |
fluG701; veA1 | C | C | C | C | FA | FA | FA | ||
veA1 suppressor | fluG10sveA1 | fluG10sveA1; veA+ | C | C | ND | A | A | A | A |
fluG20sveA1 | fluG10sveA1; veA1 | C | C | ND | FA | FA | FA | FA | |
fluG30sveA1 | |||||||||
Temperature sensitive | fluG102 | fluG102; veA+ | C | C | ND | A | FA | FA | FA |
fluG684 | fluG102, veA1 | C | C | ND | C | FA | FA | FA | |
Null | ΔfluG | ΔfluG; veA+ | FA | FA | ND | FA | FA | FA | FA |
ΔfluG; veA1 | FA | FA | ND | FA | FA | FA | FA | ||
Wild type | fluG+ | fluG+; veA+ | C | C | C | A | C | C | C |
fluG+; veA1 | C | C | C | C | C | C | C |
Colonies were incubated either in white light (WL; 35 μE m−2 sec−1), red light (RL; 610 to 720 nm; 20 μE m−2 sec−1), blue light (BL; 436 nm; 5 μE m−2 sec−1), or the dark (D). All phenotypes were examined on complete medium. 42° in blue light was not done. The fluG701 and wild-type veA+ strains contain the bliA1 allele. The symbols represent the phenotype of colonies possessing the indicated allele. C, conidial; A, aconidial; FA, aconidial with fluffy, profuse aerial hyphae; ND, not done.
mutation results from a G to A transition at nucleotide position 1739 that changes Lys467 to Glu. The identities of fluG10sveA1, fluG20sveA1, and fluG30sveA1 were determined by PCR amplification followed by DNA sequencing of genomic DNA from those selected regions shown to contain each mutation. Figure 2B indicates that fluG-10sveA1 is caused by a T to G transversion mutation at nucleotide position 1032 that converts Leu248 to a stop codon. The fluG20sveA1 mutation is caused by a T to C transition that changes Leu570 to Ser. The fluG30sveA1 mutation is caused by a T to C transition that abolishes the normal termination codon such that translation continues to the next stop codon. This results in the addition of two amino acids, arginine and valine, to the carboxyl-terminal end of the FLUG protein.
Transcription of fluG in wild-type and mutant strains: Lee and Adams (1994a) showed that fluG transcription is constitutive and not developmentally regulated in veA1 strains. We obtain the same results in a veA+ strain (data not shown). Relatively constant levels of fluG transcripts are observed throughout development regardless of whether cultures are incubated in white light, red light, or the dark. In addition, Lee and Adams (1994a) demonstrated that disruption of the fluG gene results in increased accumulation of altered fluG transcripts, indicating that fluG expression is negatively autoregulated.
We observe increased accumulation of fluG transcripts in vegetative (submerged) cultures in fluG mutations that suppress the veA1 phenotype, but we do not detect increased accumulation of transcripts in the fluG701 mutant (Figure 3A). Because the fluG701 phenotype is only detectable in surface-grown cultures exposed to red light, we examined transcript accumulation in this mutant under this restrictive condition. As shown in Figure 3B, increased accumulation of mutant transcripts is still not detectable. Furthermore, we note that fluG transcript accumulation is not affected by the allelic state of the veA gene. Although changes in light-dependent activities are observed among different fluG and veA alleles, negative autoregulation of fluG transcription does not appear to be a light-related event.
Production of and response to the extracellular factor is affected by different strains and growth conditions: Conidiation can be rescued in a fluG deletion mutant by growing the mutant adjacent to a wild-type strain (Lee and Adams 1994a). In this experiment, both the strain that served as the producer of the extracellular factor and the recipient fluG deletion strain were genotypically veA1. We first sought to determine whether the allelic state of veA affects production of the extracellular factor by growing veA+ and veA1 strains next to a ΔfluG; veA1 strain. As shown in Figure 4, A and B, both veA+ and veA1 strains are able to rescue conidiation in the yellow-spored deletion strain if incubation occurs in white light. However, if incubation occurs in the dark, a condition that suppresses conidiation in veA+ strains, production of the factor is only observed in the veA1 strain and not in the veA+ strain (Table 4).
Lee and Adams (1994a) demonstrated that production
Genotype of producing strain . | Illumiation condition . | Rescue of conidiation in the ΔfluG; veA1 recipient strain . |
---|---|---|
veA+ | White light | + |
veA+ | Red light | + |
veA+ | Dark | 0 |
veA1 | White light | + |
veA1 | Dark | + |
fluG701; veA+ | White light | + |
fluG701; veA+ | Red light | 0 |
fluG701; veA+ | Dark | 0 |
fluG10sveA1; veA1 | White light | 0 |
fluG10sveA1; veA1 | Dark | 0 |
fluG20sveA1; veA1 | White light | 0 |
fluG20sevA1; veA1 | Dark | 0 |
fluG30sveA1; veA1 | White light | 0 |
fluG30sveA1; veA1 | Dark | 0 |
Genotype of producing strain . | Illumiation condition . | Rescue of conidiation in the ΔfluG; veA1 recipient strain . |
---|---|---|
veA+ | White light | + |
veA+ | Red light | + |
veA+ | Dark | 0 |
veA1 | White light | + |
veA1 | Dark | + |
fluG701; veA+ | White light | + |
fluG701; veA+ | Red light | 0 |
fluG701; veA+ | Dark | 0 |
fluG10sveA1; veA1 | White light | 0 |
fluG10sveA1; veA1 | Dark | 0 |
fluG20sveA1; veA1 | White light | 0 |
fluG20sevA1; veA1 | Dark | 0 |
fluG30sveA1; veA1 | White light | 0 |
fluG30sveA1; veA1 | Dark | 0 |
The AfluG; veA1 strain, TTA 127.4, was grown adjacent to either a veA+ strain (FGSC 4), a veA1 strain (TU 1), the fluG701 mutant (TU 110), the fluG10sveA1 mutant (TU39), the fluG20sveA1 mutant (TU 41), or the fluG30sveA1 mutant (TU 42). All incubations were performed on complete medium at 32° in either full spectrum white light (35 μE m−2 sec−1), red light (610 to 720 nm; 20 μE m−2sec−1) or the dark. Cultures were examined between 48 and 72 hr for the ability of thte producing strain to rescue conidiation in the AfluG; veA1 strain.
Genotype of producing strain . | Illumiation condition . | Rescue of conidiation in the ΔfluG; veA1 recipient strain . |
---|---|---|
veA+ | White light | + |
veA+ | Red light | + |
veA+ | Dark | 0 |
veA1 | White light | + |
veA1 | Dark | + |
fluG701; veA+ | White light | + |
fluG701; veA+ | Red light | 0 |
fluG701; veA+ | Dark | 0 |
fluG10sveA1; veA1 | White light | 0 |
fluG10sveA1; veA1 | Dark | 0 |
fluG20sveA1; veA1 | White light | 0 |
fluG20sevA1; veA1 | Dark | 0 |
fluG30sveA1; veA1 | White light | 0 |
fluG30sveA1; veA1 | Dark | 0 |
Genotype of producing strain . | Illumiation condition . | Rescue of conidiation in the ΔfluG; veA1 recipient strain . |
---|---|---|
veA+ | White light | + |
veA+ | Red light | + |
veA+ | Dark | 0 |
veA1 | White light | + |
veA1 | Dark | + |
fluG701; veA+ | White light | + |
fluG701; veA+ | Red light | 0 |
fluG701; veA+ | Dark | 0 |
fluG10sveA1; veA1 | White light | 0 |
fluG10sveA1; veA1 | Dark | 0 |
fluG20sveA1; veA1 | White light | 0 |
fluG20sevA1; veA1 | Dark | 0 |
fluG30sveA1; veA1 | White light | 0 |
fluG30sveA1; veA1 | Dark | 0 |
The AfluG; veA1 strain, TTA 127.4, was grown adjacent to either a veA+ strain (FGSC 4), a veA1 strain (TU 1), the fluG701 mutant (TU 110), the fluG10sveA1 mutant (TU39), the fluG20sveA1 mutant (TU 41), or the fluG30sveA1 mutant (TU 42). All incubations were performed on complete medium at 32° in either full spectrum white light (35 μE m−2 sec−1), red light (610 to 720 nm; 20 μE m−2sec−1) or the dark. Cultures were examined between 48 and 72 hr for the ability of thte producing strain to rescue conidiation in the AfluG; veA1 strain.
of the extracellular factor is dependent on a functional fluG gene. Not surprisingly, the fluG701 mutant produces the factor only in white light, which is the permissive condition that allows conidiation in this mutant (Table 4). However, the veA1 suppressors all fail to produce the extracellular factor in conditions that promote conidiation (surface-grown cultures exposed to white light; Table 4).
We next inquired whether the allelic state of veA affects the ability of a fluG deletion strain to respond to the extracellular factor. As shown in Figure 4, C and D, rescue is not observed in the yellow-spored ΔfluG; veA+ strain regardless of whether it is grown adjacent to a veA+ or veA1 strain and despite the fact that all cultures are exposed to full spectrum white light.
DISCUSSION
Light is an important environmental stimulus that can elicit conidiation in wild-type strains of A. nidulans. We describe the isolation and characterization of two new classes of fluG mutant alleles that display abnormal responses to light. One class is defined by a mutation that causes failure to conidiate in red light in a veA+ strain that is normally red light responsive. The other class includes mutations that restore red light-dependent conidiation to the veA1 mutant that normally conidiates without dependence on red light. The fluG gene encodes a polypeptide having unknown function that shares limited identity to GSI-type prokaryotic glutamine synthetases (Lee and Adams 1994a). The identification of fluG mutant alleles that affect responses to light suggests the possibility that fluG encodes the red light photoreceptor. At the very least, the identification of these two mutant classes indicates that fluG activity influences and/or is affected by light-dependent functions.
Lee and Adams (1994a) showed that the fluG gene is involved in the production of an extracellular factor that is necessary for the activation of the major programmed pathway of asexual development. Since the FLUG polypeptide is constitutively expressed in both vegetative and developing cultures, they proposed that the extracellular factor accumulates independent of environmental conditions and that conidiation is induced only after a threshold level of the sporulation-inducing factor is achieved. This hypothesis was suggested by experiments performed in strains containing the veA1 mutation. However, the inability of fluG+; veA+ strains to produce extracellular factor in the absence of light, despite constitutive expression of fluG transcription, suggests that environmental conditions may influence the production of the factor. Lee and Adams (1994a) have shown that the absence of conidiation is not sufficient to inhibit synthesis of the extracellular factor, because both aconidial brlA deletion and flbA deletion strains are able to rescue conidiation in a fluG deletion. Thus, the failure of veA+ strains to produce the factor in the dark could indicate that light-dependent veA activities either precede or are required for those fluG functions associated with synthesis of the extracellular signal.
Although the FLUG polypeptide shares significant homology with GSI-type prokaryotic glutamine synthetases, Lee and Adams (1994a) indicated that it is unlikely that FLUG has glutamine synthetase activity. Rather, it is possible that FLUG has a related enzymatic activity that is responsible for the production of the extracellular factor. We have shown, not unexpectedly, that production of the extracellular factor requires that a strain contain a wild-type fluG allele or an allele that produces a functional gene product under permissive conditions. Strains containing a disrupted gene do not produce the factor. GSI homologous sequences are located in the carboxyl terminal 436 amino acids. This region shares 28% identity and 50% similarity with GSI from Bacillus cereus. The fluG701, fluG20sveA1, and fluG30sveA1 mutations are all located within the GSI homologous region, suggesting that these mutations may be disrupting the postulated enzymatic activity of FLUG. Although the fluG10sveA1 mutation is located upstream of the GSI homologous domain, its identity as a nonsense mutation is consistent with the disrupted enzymatic activity of fluG.
The perception of the extracellular signal does not require fluG function, as evidenced by phenotypic complementation of a ΔfluG; veA1 strain when inoculated adjacent to either veA+ or veA1 strains. But why can't conidiation be rescued in ΔfluG; veA+ strains? An attractive explanation is that signal perception may be repressed in a veA+ strain and that derepression requires fluG activity. The possibility for such an interaction between these gene products is supported by the identification of mutations in fluG that suppress the veA1 phenotype. However, the possibility that veA+ strains are simply unable to respond to the sporulation-inducing factor regardless of which fluG allele is present cannot be excluded, though it would seem unlikely that veA+ strains that produce this factor would be constitutively unable to respond to it.
It is clear that fluG participates in the synthesis of the extracellular factor, but it is also apparent that fluG influences light-dependent activities associated with conidiation. Examples of fluG's effect on light-dependent conidiation include the loss of red-light perception in the fluG701 mutant and the identification of specific fluG mutations that suppress the veA1 mutation. Although Lee and Adams (1994a) suggest that the enzymatic domain of FLUG involved with production of the extracellular factor lies within the carboxyl-terminal 436 amino acid, it is unclear what portion of the molecule participates in light-dependent functions. Since the fluG10sveA1 mutation introduces a stop codon at amino acid position 248, it is possible that the amino-terminal end of FLUG functions in light-related activities. However, a strain bearing a fluG mutant allele that encodes a truncated form of FLUG containing only its first 250 amino acids is phenotypically different from fluG10sveA1 and does not suppress the veA1 mutation (unpublished data). The reasons for this difference are not clear at present, but it is possible that the phenotype of fluG-10sveA1 arises not from high-level production of a truncated polypeptide but from low-level production of an altered full-length polypeptide, e.g., through low-level read-through of the premature termination codon by nonsense codon suppression.
The identification of fluG mutations that suppress the veA1 phenotype suggests a genetic interaction between these two genes. A simple model to explain the relationship between the fluG and veA is that veA functions as an integrator of the light signal and modulates the expression of fluG, thereby regulating the extracellular signal. Mooney and Yager (1990) speculated that veA functions as a negative regulator of conidiation-specific gene expression. Thus, as suggested above, the expression of fluG could be derepressed in veA1 strains. Alternatively, an interaction between FLUG and VEA might be necessary to effect fluG function. Other known signal integrators control the transcriptional activity of down-stream regulatory elements (Bradford et al. 1996; Kamei et al. 1996). However, neither light nor the allelic state of veA appears to affect the level of fluG mRNA, suggesting that, if VEA has effects on fluG expression, then these effects are posttranscriptional.
It is unlikely that veA and fluG form a strict linear pathway. The ability of the extracellular factor to rescue conidiation in a ΔfluG; veA1 strain, but not in a ΔfluG; veA+ strain, suggests that veA activity could be also affecting the expression of downstream conidiation-specific genes. For example, veA could act as a general repressor of certain developmental functions. Downstream gene activation would require derepression by the veA signal integrator followed by initiation of functions that are regulated by the fluG signal. In a veA1 strain, this repression is absent, and downstream gene activation requires only that these functions be initiated by the fluG signal. This dual activation mechanism would insure that conidiation proceeds only in response to correct environmental and internal preprogrammed signals.
Although certain developmental events occur in response to genetically preprogrammed signals, the initiation of conidiation is also responsive to specific external stimuli. Light appears to influence the activities of both the FLUG and VEA gene products. Conversely, the activities of these genes may also be affecting other light-related events. An understanding of how veA and fluG influence each other's function and how these two genes coordinate the activities of other conidiation-specific genes remain important questions to be solved.
Acknowledgement
We are grateful to Thomas Adams for providing strains, plasmids and oligonucleotides and for allowing J.E.Z. to visit his laboratory to learn the cycle-sequencing procedure. We also thank Greg May and Sewell P. Champe for providing strains used in this study, Richard Waring for critical reading of this manuscript, and Richard Friedman for his assistance with the computer-generated illustrations. This work was supported by National Science Foundation grant IBN9219031 to L.N.Y.
Footnotes
Communicating editor: J. J. Loros
LITERATURE CITED
Author notes
These authors contributed equally to this work.
Present address: Millennium Pharmaceuticals, Inc., 640 Memorial Drive, Cambridge, MA 02139.
Present address: Department of Biology, University of Pennsylvania, Philadelphia, PA 19104.