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Alan B Rose, Jiayang Li, Robert L Last, An Allelic Series of Blue Fluorescent trp1 Mutants of Arabidopsis thaliana, Genetics, Volume 145, Issue 1, 1 January 1997, Pages 197–205, https://doi.org/10.1093/genetics/145.1.197
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Nine blue fluorescent mutants of the flowering plant Arabidopsis thaliana were isolated by genetic selections and fluorescence screens. Each was shown to contain a recessive allele of trp1, a previously described locus that encodes the tryptophan biosynthetic enzyme phosphoribosylanthranilate transferase (PAT, called trpD in bacteria). The trp1 mutants consist of two groups, tryptophan auxotrophs and prototrophs, that differ significantly in growth rate, morphology, and fertility. The trp1 alleles cause plants to accumulate varying amounts of blue fluorescent anthranilate compounds, and only the two least severely affected of the prototrophs have any detectable PAT enzyme activity. All four of the trp1 mutations that were sequenced are G to A or C to T transitions that cause an amino acid change, but in only three of these is the affected residue phylogenetically conserved. There is an unusually high degree of sequence divergence in the single-copy gene encoding PAT from the wild-type Columbia and Landsberg erecta ecotypes of Arabidopsis.
Communicating editor: K. J. Newton
Author notes
Present address: Section of Molecular and Cellular Biology, University of California, Davis, CA 95616-8535.
Present address: Institute of Genetics, Academia Sinica, Beijing 100101, China.