Thirty-five period locus sequences from Drosophila pseudoobscura and its siblings species, D. p. bogotana, D. persirnilis, and D. miranda, were studied. A large amount of variation was found within D. pseudoobscura and D. persimilis, consistent with histories of large effective population sizes. D. p. bogotana, however, has a severe reduction in diversity. Combined analysis of per with two other loci, in both D. p. bogotana and D. pseudoobscura, strongly suggest this reduction is due to recent directional selection at or near per within D. p. bogotana. Since D. p. bogotana is highly variable and shares variation with D. pseudoobscura at other loci, the low level of variation at per within D. p. bogotana can not be explained by a small effective population size or by speciation via founder event. Both D. pseudoobscura and D. persirnilis have considerable intraspecific gene flow. A large portion of one D. persirnilis sequence appears to have arisen via introgression from D. pseudoobscura. The time of this event appears to be well after the initial separation of these two species. The estimated times since speciation are one mya for D. pseudoobscura and D. persirnilis and 2 mya since the formation of D. miranda.
- Received June 3, 1996.
- Accepted August 3, 1996.
- Copyright © 1996 by the Genetics Society of America