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Tracey Chapman
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doi:10.1534/genetics.107.083766
A more recent version of this article appeared on March 1, 2008.
REGULAR RESEARCH PAPERS |
Targeted gene deletion and phenotypic analysis of the Drosophila melanogaster seminal fluid protease inhibitor Acp62F
Jacob L. Mueller 1, Jon Linklater 2, Kristipati Ravi Ram 1, Tracey Chapman 3 and Mariana F. Wolfner 1*
1 Cornell University
2 University College, London UK
3 University of East Anglia, UK
* To whom correspondence should be addressed. E-mail: mfw5{at}cornell.edu.
Submitted on October 24, 2007
Revised on December 6, 2007
Accepted on 4 January 2008
Internally fertilizing organisms transfer a complex assortment of seminal fluid proteins, a substantial fraction of which are proteolysis regulators. In mammals, some seminal protease inhibitors have been implicated in male infertility and these same molecular classes of protease inhibitors are also found in Drosophila seminal fluid. Here, we tested the reproductive functions of the Drosophila melanogaster seminal fluid protease inhibitor Acp62F by generating a precise deletion of the Acp62F gene. We did not detect a non-redundant function for Acp62F in modulating the egg-laying, fertility, remating frequency or lifespan of mated females. However, loss of Acp62F did alter a male's defensive sperm competitive ability, consistent with the localization of Acp62F to sperm storage organs. In addition, the processing of at least one seminal protein, the ovulation hormone ovulin, is slower in the absence of Acp62F.
Key Words: lifespan, male accessory gland proteins, protease inhibitor, reproduction, sperm competition
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