Genetics. Published Articles Ahead of Print: October 18, 2007, Copyright © 2007
doi:10.1534/genetics.107.078360


A more recent version of this article appeared on November 1, 2007.

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A mosaic genetic screen for Drosophila neoplastic tumor suppressor genes based on defective pupation

Laurent Menut 1, Thomas Vaccari 1, Heather Dionne 1, Joseph Hill 1, Geena Wu 1 and David Bilder 1*

1 University of California, Berkeley

* To whom correspondence should be addressed. E-mail: bilder{at}berkeley.edu.

Submitted on July 3, 2007
Revised on August 16, 2007
Accepted on 31 August 2007


Abstract

The Drosophila neoplastic tumor suppressor genes (TSGs) coordinately control cell polarity and proliferation in epithelial and neuronal tissues. While a small group of neoplastic TSG mutations have been isolated and their corresponding genes cloned, the regulatory pathways that normally prevent inappropriate growth remain unclear. Identification of additional neoplastic TSGs may provide insight into this question. We report here the design of an efficient screen to isolate neoplastic TSG mutations utilizing genetically mosaic larvae. This screen is based on a defective pupation phenotype seen when a single pair of imaginal discs is homozygous for a neoplastic TSG mutation, which suggests that continuously proliferating cells can interfere with metamorphosis. Execution of this screen on two chromosome arms led to the identification of mutations in at least seven new neoplastic TSGs. The isolation of additional loci that affect hyperplastic as well as neoplastic growth indicates the utility of this screening strategy for studying epithelial growth control.

Key Words: Drosophila, epithelial polarity, genetic screen, pupation, tumor suppressor gene