Genetics. Published Articles Ahead of Print: July 29, 2007, Copyright © 2007
doi:10.1534/genetics.107.078147


A more recent version of this article appeared on October 1, 2007.

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High density detection of restriction site associated DNA (RAD) markers for rapid mapping of mutated loci in Neurospora

Zachary A Lewis 1, Anthony L Shiver 1, Nicholas Stiffler 1, Michael R Miller 1, Eric A Johnson 1 and Eric U Selker 1*

1 University of Oregon

* To whom correspondence should be addressed. E-mail: selker{at}molbio.uoregon.edu.

Submitted on June 25, 2007
Revised on July 13, 2007
Accepted on 24 July 2007


Abstract

The wealth of sequence information available for Neurospora crassa and other fungi has greatly facilitated evolutionary and molecular analyses of this group. Although "reverse" genetics, in which genes are first identified by their sequence rather than by their mutant phenotypes, serves as a valuable new approach to elucidate biological processes, classical "forward" genetic analysis is still extremely useful. Unfortunately, mapping mutations and identifying the corresponding genes has typically been slow and laborious. To facilitate forward genetics in Neurospora, we have adapted microarray-based restriction site associated DNA (RAD) mapping for use with N. crassa oligonucleotide microarrays. This technique was used to simultaneously detect an unprecedented number of genome wide restriction site polymorphisms from two Neurospora crassa strains: Mauriceville and Oak Ridge. Furthermore, RAD mapping was used to quickly map a previously unknown gene, defective in methylation-7 (dim-7).

Key Words: DNA methylation, Microarray, Neurospora, genetic map, restriction site associated DNA (RAD)