A genetic screen for DNA double strand break repair mutations in Drosophila

The study of DNA double strand break (DSB) repair has been greatly facilitated by the use of rare-cutting endonucleases, which induce a break precisely at their cut sites that can be strategically placed in the genome. We previously established such a system in Drosophila and showed that the yeast I-SceI enzyme cuts efficiently in Drosophila cells and those breaks are effectively repaired by conserved mechanisms. In this study, we determined the genetic requirements for the repair of this I-SceI-induced DSB in the germline. We show that Drosophila Rad51 and Rad54 are both required for homologous repair by gene conversion, but are dispensable for single strand annealing repair. We provided evidence suggesting that Rad51 is more stringently required than Rad54 for inter-sister gene conversion. We uncovered a significant role of DNA ligase IV in non-homologous end joining. We conducted a screen for candidate mutations affecting DSB repair, and discovered novel mutations in genes that include mutagen sensitive 206, single strand annealing reducer and others. In addition, we demonstrated an intricate balance among different repair pathways in which the cell differentially utilizes repair mechanisms in response to both changes in the genomic environment surrounding the break and deficiencies in one or the other repair pathways.

Key Words: I-SceI, drosophila ligase 4, homologous recombination, inter-sister recombination, nhej

Online ISS 1091-6490
Copyright 2008 by the Genetics Society of America
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