Interphase chromosome arrangement in Arabidopsis thaliana is similar between differentiated and meristematic tissues and shows a transient mirror symmetry after nuclear division

Whole-mount fluorescent in situ hybridization (FISH) was applied to Arabidopsis thaliana seedlings to determine the three-dimensional (3D) interphase chromosome territory (CT) arrangement and heterochromatin location within the positional context of entire tissues or in particular cell types of morphologically well preserved seedlings. The interphase chromosome arrangement was found to be similar between all inspected meristematic and differentiated root and shoot cells indicating a lack of a gross reorganization during differentiation. The predominantly random CT arrangement (except for a more frequent association of the homologous chromosomes bearing a nucleolus organizer) and the peripheric location of centromeric heterochromatin were as previously observed for flow-sorted nuclei, but centromeres tend to fuse more often in non-endoreduplicating cells and NORs in differentiated cells. After mitosis, sister nuclei revealed a symmetric arrangement of homologous CTs waning with the progress of the cell cycle or in the course of differentiation. Thus, the interphase chromosome arrangement in A. thaliana nuclei seems to be constrained mainly by morphological features such as nuclear shape, presence or absence of a nucleolus organizer on chromosomes, nucleolar volume and/or endopolyploidy level.

Key Words: Arabidopsis thaliana, whole-mount fluorescence in situ hybridization (FISH) and chromosome painting,

This article has been cited by other articles:

				T. A. Dittmer, N. J. Stacey, K. Sugimoto-Shirasu, and E. J. Richards LITTLE NUCLEI Genes Affecting Nuclear Morphology in Arabidopsis thaliana PLANT CELL, September 1, 2007; 19(9): 2793 - 2803. [Abstract] [Full Text] [PDF]

Online ISS 1091-6490
Copyright 2008 by the Genetics Society of America
phone: 412-268-1812   fax: 412-268-1813   email: genetics-gsa{at}andrew.cmu.edu