Genetics. Published Articles Ahead of Print: May 4, 2007, Copyright © 2007
doi:10.1534/genetics.107.072983


A more recent version of this article appeared on June 1, 2007.

NOTE

Manipulating mitotic recombination in the zebrafish embryo through RecQ helicases

Jing Xie 1, Seneca L. Bessling 1, Timothy K. Cooper 1, Harry C. Dietz 1, Andrew S. McCallion 1 and Shannon Fisher 1*

1 Johns Hopkins University School of Medicine

* To whom correspondence should be addressed. E-mail: sfisher{at}jhmi.edu.

Submitted on March 8, 2007
Revised on March 25, 2007
Accepted on 16 April 2007


Abstract

RecQ DNA helicases resolve Rad-51-mediated recombination and suppress aberrant homologous recombination. RecQ gene loss is associated with cancer susceptibility and increased mitotic recombination. We have developed an in vivo assay based on a zebrafish pigment mutant for suppression of RecQ activity, and demonstrate that zebrafish RecQ genes have conserved function in suppressing mitotic recombination.

Key Words: RecQ helicase, blm, mitotic recombination, recql5, zebrafish