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genetics.107.071092v1
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doi:10.1534/genetics.107.071092
A more recent version of this article appeared on May 1, 2007.
REGULAR RESEARCH PAPERS |
Differential Epigenetics Regulation Within an Arabidopsis Retroposon Family
Sanjida H. Rangwala 1 and Eric J. Richards 1*
1 Washington University
* To whom correspondence should be addressed. E-mail: richards{at}biology2.wustl.edu.
Submitted on January 17, 2007
Revised on February 20, 2007
Accepted on 20 February 2007
We previously reported a novel family of Arabidopsis thaliana non-autonomous retroposons, Sadhu, featuring epigenetic variation in natural populations. Here, we show that Sadhu elements are re-expressed in a subset of mutants carrying disruptions in genes encoding chromatin modification enzymes, but are not significantly expressed in mutants defective in RNA silencing pathways, indicating that RNA-directed processes are not necessary to maintain transcriptional suppression of this class of retroelements. We focused our analysis on three representative elements showing differential responses to ddm1, met1, and hda6 mutations. These mutations had differing effects on cytosine methylation depending on the element and the sequence context. Curiously, the Sadhu6-1 element with strongest CpHpG methylation is re-expressed only in a met1 CpG methyltransferase mutant, and is not affected by ddm1 or cmt3 mutations. Regardless of the mutant background, H3meK9 was found at silenced loci, while H3meK4 was restricted to expressed alleles. We discuss the different modes of regulation within this family and the potential impact of this regulation on the stability of silencing in natural populations.
Key Words: Arabidopsis, Chromatin, DNA methylation, Epigenetics, Retroelements
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