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doi:10.1534/genetics.107.070904
A more recent version of this article appeared on May 1, 2007.
REGULAR RESEARCH PAPERS |
Transcriptional Control of Gluconeogenesis in Aspergillus nidulans
Michael John Hynes 1*, Edyta Szewczyk 2, Sandra Louise Murray 1, Yumi Suzuki 1, Meryl Anne Davis 1 and Heather Sealy-Lewis 3
1 University of Melbourne
2 State University of Ohio
3 University of Hull
* To whom correspondence should be addressed. E-mail: mjhynes{at}unimelb.edu.au.
Submitted on January 13, 2007
Revised on February 9, 2007
Accepted on 16 February 2007
Aspergillus nidulans can utilise carbon sources that result in the production of TCA cycle intermediates therefore requiring gluconeogenesis. We have cloned the acuG gene encoding fructose-1,6 bisphosphatase and found that expression of this gene is regulated by carbon catabolite repression as well as by induction by a TCA cycle intermediate similar to the induction of the previously studied acuF gene encoding phospho-enol-pyruvate carboxykinase. The acuN356 mutation results in loss of growth on gluconeogenic carbon sources. Cloning of acuN has shown that it encodes enolase, an enzyme involved in both glycolysis and gluconeogenesis. The acuN356 mutation is a translocation with a breakpoint in the 5' untranslated region resulting in loss of expression in response to gluconeogenic but not glycolytic carbon sources. Mutations in the acuK and acuM genes affect growth on carbon sources requiring gluconeogenesis and result in loss of induction of the acuF, acuN and acuG genes by sources of TCA cycle intermediates. Isolation and sequencing of these genes has shown that they encode proteins with similar but distinct Zn(2) Cys(6) DNA binding domains suggesting a direct role in transcriptional control of gluconeogenic genes. These genes are conserved in other filamentous ascomycetes indicating their significance for the regulation of carbon source utilisation.
Key Words: carbon metabolism, enolase, filamentous fungi, gene regulation, transcription factor
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