- THIS ARTICLE
- Full Text (Rapid PDF)
-
All Versions of this Article:
genetics.107.070771v1
177/3/1527 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Hatin, I.
- Articles by Rousset, J.-P.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Hatin, I.
- Articles by Rousset, J.-P.
doi:10.1534/genetics.107.070771
A more recent version of this article appeared on November 1, 2007.
REGULAR RESEARCH PAPERS |
Fine tuning of translation termination efficiency in Saccharomyces cerevisiae involves two factors in close proximity to the exit tunnel of the ribosome
Isabelle Hatin 1*, Celine Fabret 1, Olivier Namy 1, Wayne Decatur 2 and Jean-Pierre Rousset 1
1 IGM
2 University of Massachusetts
* To whom correspondence should be addressed. E-mail: isabelle.hatin{at}igmors.u-psud.fr.
Submitted on January 11, 2007
Revised on March 5, 2007
Accepted on 27 April 2007
In eukaryotes, release factor 1 and 3 (eRF1 and eRF3) are recruited to promote translation termination when a stop codon on the mRNA enters at the ribosomal A-site. However, their over-expression increases only moderately termination efficiency, suggesting that other factors might be involved in the termination process. In order to determine such unknown components, we performed a genetic screen in Saccharomyces cerevisiae that identified genes increasing termination efficiency when over-expressed. For this purpose, we constructed a dedicated reporter strain in which a leaky stop codon is inserted into the chromosomal copy of the ade2 gene. Twenty-five anti-suppressor candidates were identified and characterized for their impact on readthrough. Among them, SSB1 and snR18, two factors close to the exit tunnel of the ribosome, directed the strongest anti-suppression effects when over-expressed, showing that they may be involved in fine tuning of the translation termination level.
Key Words: SSB chaperones, eEF1B, snoRNA, translation termination
This article has been cited by other articles:
 |
|
 |
|
  L. A. Strawn, C. A. Lin, E. M.H. Tank, M. M. Osman, S. A. Simpson, and H. L. True Mutants of the Paf1 Complex Alter Phenotypic Expression of the Yeast Prion [PSI+] Mol. Biol. Cell, April 15, 2009; 20(8): 2229 - 2241. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Hatin, C. Fabret, J.-P. Rousset, and O. Namy Molecular dissection of translation termination mechanism identifies two new critical regions in eRF1 Nucleic Acids Res., April 1, 2009; 37(6): 1789 - 1798. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Conz, H. Otto, K. Peisker, M. Gautschi, T. Wolfle, M. P. Mayer, and S. Rospert Functional Characterization of the Atypical Hsp70 Subunit of Yeast Ribosome-associated Complex J. Biol. Chem., November 23, 2007; 282(47): 33977 - 33984. [Abstract] [Full Text] [PDF]
|
|
|