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doi:10.1534/genetics.106.069781
A more recent version of this article appeared on June 1, 2007.
REGULAR RESEARCH PAPERS |
The Early Developmental Gene Semaphorin 5c Contributes to Olfactory Behavior in Adult Drosophila
Stephanie M Rollmann 1, Akihiko Yamamoto 1, Tim Goossens 2, Liesbeth Zwarts 2, Zsuzsanna Callaerts-Végh 2, Patrick Callaerts 2, Koenraad Norga 2, Trudy F C Mackay 1 and Robert R H Anholt 1*
1 North Carolina State University
2 University of Leuven
* To whom correspondence should be addressed. E-mail: anholt{at}ncsu.edu.
Submitted on December 15, 2006
Revised on February 12, 2007
Accepted on 13 April 2007
Behaviors are complex traits influenced by multiple pleiotropic genes. Understanding the mechanisms that give rise to complex behaviors requires an understanding of how variation in transcriptional regulation shapes nervous system development and how variation in brain structure influences an organism's ability to respond to its environment. To begin to address this problem we have used olfactory behavior in Drosophila melanogaster as a model and showed that a hypomorphic transposon-mediated mutation of the early developmental gene Semaphorin-5c (Sema-5c) results in aberrantt behavioral responses to the repellant odorant benzaldehyde. We fine mapped this effect to the Sema-5c locus using deficiency mapping, phenotypic reversion through P-element excision, and transgenic rescue. Morphometric analysis of this Sema-5c allele reveals subtle neuroanatomical changes in the brain with a reduction in the size of the ellipsoid body. High-density oligonucleotide expression microarrays identified 50 probe sets with altered transcriptional regulation in the Sema-5c background and quantitative complementation tests identified epistatic interactions between nine of these co-regulated genes and the transposon-disrupted Sema-5c gene. Our results demonstrate how hypomorphic mutation of an early developmental gene results in genome-wide transcriptional consequences and alterations in brain structure accompanied by profound impairment of adult behavior.
Key Words: behavioral genetics, neuroanatomy, neurodevelopment, olfaction, transcriptional profiling
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