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doi:10.1534/genetics.106.069666
A more recent version of this article appeared on May 1, 2007.
NOTE |
An Unusual Pattern of Spontaneous Mutations Recovered in the Halophilic Archaeon Haloferax volcanii
Reena R. Mackwan 1, Geraldine T. Carver 2, John W. Drake 2 and Dennis Grogan 1*
1 University of Cincinnati
2 National Institute of Environmental Health Sciences
* To whom correspondence should be addressed. E-mail: grogandw{at}email.uc.edu.
Submitted on December 13, 2006
Revised on December 14, 2006
Accepted on 14 December 2006
Spontaneous mutations in the orotate:phosphoribosyl transferase (pyrE2) gene of the halophilic archaeon Haloferax volcanii were selected by 5-fluoro-orotic acid plus uracil at a rate of about 2 x 10-8 per cell division in fluctuation and null-fraction tests but about 6 x 10-8 per cell division in mutation-accumulation tests. The corresponding genomic mutation rates were substantially lower than observed for other mesophilic microbial DNA genomes based on similar target genes. The mutational spectrum was dominated by indels adding or deleting multiples of three base-pairs. Properties of the organism contributing to this unusual mutational pattern may include phenotypic lag caused by a high chromosomal copy number and efficient promotion of strand misalignments by short direct repeats.
Key Words: Haloferax volcanii, halophilic archaea, mutation rate, mutation spectrum, mutation, spontaneous
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