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doi:10.1534/genetics.106.068270
A more recent version of this article appeared on March 1, 2007.
REGULAR RESEARCH PAPERS |
Circadian Rhythms in Neurospora crassa: Clock mutant effects in the absence of a frq-based oscillator
Laura M Lombardi 1, Kevin Schneider 1, Michelle Tsukamoto 1 and Stuart Brody 1*
1 UCSD
* To whom correspondence should be addressed. E-mail: sbrody{at}ucsd.edu.
Submitted on November 17, 2006
Revised on December 21, 2006
Accepted on 4 January 2007
In Neurospora, the circadian rhythm is expressed as rhythmic conidiation driven by a feedback loop involving the protein products of frq (frequency), wc-1 (white collar-1), and wc-2, known as the frq/wc (FWC) oscillator. Although strains carrying null mutations such as frq10 or wc-2
lack a functional FWC oscillator and do not show a rhythm under most conditions, a rhythm can be observed in them by the addition of geraniol or farnesol to the media. Employing this altered media as an assay, the effect of other clock mutations in a frq10 or wc-2 null background can be measured. It was found that the existing clock mutations fall into three classes: 1) those, such as prd-3 or prd-4 or frq1 that showed no effect in a clock null background; 2) those, such as prd-1 or prd-2 or prd-6, that did have a measurable effect in the frq10 background; and 3) those, such as the new mutation ult, that suppressed the frq10 or wc-2 effect, i.e. geraniol/farnesol were not required for a visible rhythm. This classification suggests that some of the known clock mutations are part of a broader multi-oscillator system.
Key Words: Neurospora, circadian rhythms, frq-less oscillator, prd, white collar
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