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doi:10.1534/genetics.106.068247
A more recent version of this article appeared on March 1, 2007.
REGULAR RESEARCH PAPERS |
Evolution of different Y chromosomes in two medaka species, Oryzias dancena and O. latipes
Yusuke Takehana 1*, Diana Demiyah 1, Kiyoshi Naruse 2, Satoshi Hamaguchi 1 and Mitsuru Sakaizumi 1
1 Niigata University
2 University of Tokyo
* To whom correspondence should be addressed. E-mail: yusuke{at}env.sc.niigata-u.ac.jp.
Submitted on November 14, 2006
Revised on December 13, 2006
Accepted on 19 December 2006
Although the sex-determining gene DMY has been identified on the Y chromosome in the medaka (Oryzias latipes), this gene is absent in the most Oryzias species, suggesting that closely related species have different sex-determining genes. Here, we investigated the sex determination mechanism in Oryzias dancena, which do not possess the DMY gene. Since heteromorphic sex chromosomes have not been reported in this species, a progeny test of sex-reversed individuals produced by hormone treatment was performed. Sex-reversed males yielded all-female progeny, indicating that O. dancena has an XX/XY sex determination system. To uncover the cryptic sex chromosomes, sex-linked DNA markers were screened using expressed sequence tags (ESTs) established in O. latipes. Linkage analysis of isolated sex-linked ESTs showed a conserved synteny between the sex chromosomes in O. dancena and an autosome in O. latipes. Fluorescence in situ hybridization (FISH) analysis of these markers confirmed that sex chromosomes of these species are not homologous. These findings strongly suggest an independent origin of sex chromosomes in O. dancena and O. latipes. Further analysis of the sex-determining region in O. dancena should provide a key insight into the evolution of sex determination mechanisms in vertebrates.
Key Words: DMY, sex chromosome evolution, sex-determining gene
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