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doi:10.1534/genetics.106.067116
A more recent version of this article appeared on July 1, 2007.
REGULAR RESEARCH PAPERS |
The plexin PLX-2 and the ephrin EFN-4 have distinct roles in MAB-20/Semaphorin 2A signaling in C. elegans morphogenesis
Fumi Nakao 1, Martin L Hudson 2, Motoshi Suzuki 1, Zachary Peckler 2, Rie Kurokawa 1, Zhicen Liu 1, Keiko Gengyo-Ando 3, Akira Nukazuka 1, Takashi Fujii 1, Fumikazu Suto 1, Yukimasa Shibata 1, Go Shioi 1, Hajime Fujisawa 1, Shohei Mitani 3, Andrew D Chisholm 2 and Shin Takagi 4*
1 Nagoya University Graduate School of Science
2 University of California, Santa Cruz
3 Tokyo Women's Medical University School of Medicine
4 Nagoya University
* To whom correspondence should be addressed. E-mail: i45116a{at}nucc.cc.nagoya-u.ac.jp.
Submitted on October 21, 2006
Revised on December 9, 2006
Accepted on 24 April 2007
Semaphorins are extracellular proteins that regulate axon guidance and morphogenesis by interacting with a variety of cell surface receptors. Most semaphorins interact with plexin-containing receptor complexes, although some interact with non-plexin receptors. Class 2 semaphorins are secreted molecules that control axon guidance and epidermal morphogenesis in Drosophila and C. elegans. We show that the C. elegans class 2 semaphorin MAB-20 binds the plexin PLX-2. plx-2 mutations enhance the phenotypes of hypomorphic mab-20 alleles but not those of mab-20 null alleles, indicating that plx-2 and mab-20 act in a common pathway. Both mab-20 and plx-2 mutations affect epidermal morphogenesis during embryonic and in post-embryonic development. In both contexts plx-2 null mutant phenotypes are much less severe than mab-20 null phenotypes, indicating PLX-2 is not essential for MAB-20 signaling. Mutations in the ephrin efn-4 do not synergize with mab-20, indicating EFN-4 may act in MAB-20 signaling. EFN-4 and PLX-2 are coexpressed in the late embryonic epidermis where they play redundant roles in MAB-20 dependent cell sorting.
Key Words: C. elegans, ephrin, morphogenesis, plexin, semaphorin
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