- THIS ARTICLE
- Full Text (Rapid PDF)
-
All Versions of this Article:
genetics.106.067033v1
176/1/563 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Stich, B.
- Articles by Buckler, E. S
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Stich, B.
- Articles by Buckler, E. S
doi:10.1534/genetics.106.067033
A more recent version of this article appeared on May 1, 2007.
REGULAR RESEARCH PAPERS |
Power to detect higher-order epistatic interactions in a metabolic pathway using a new mapping strategy
Benjamin Stich 1, Jianming Yu 2, Albrecht E. Melchinger 1*, Hans-Peter Piepho 3, Friedrich Utz 1, Hans P Maurer 1 and Edward S Buckler 2
1 Institute of Plant Breeding, Seed Science, and Population Genetics; University of Hohenheim
2 Institute for Genomic Diversity; Cornell University
3 Institute for Crop Production and Grassland Research; University of Hohenheim
* To whom correspondence should be addressed. E-mail: melchinger{at}uni-hohenheim.de.
Submitted on October 20, 2006
Revised on December 8, 2006
Accepted on 20 December 2006
Epistatic interactions among quantitative trait loci (QTL) contribute substantially to the variation in complex traits. The main objectives of this study were to (i) compare three- vs. four-step genome scans to identify three-way epistatic interactions among QTL belonging to a metabolic pathway, (ii) investigate by computer simulations the power and proportion of false positives (PFP) for detecting three-way interactions among QTL in recombinant inbred line (RIL) populations derived from a nested mating design, and (iii) compare these estimates to those obtained for detecting three-way interactions among QTL in RIL populations derived from diallel and different partial diallel mating designs. The single nucleotide polymorphism haplotype data of B73 and 25 diverse maize inbreds were used to simulate the production of various RIL populations. Compared to the three-step genome scan, the power to detect three-way interactions was higher with the four-step genome scan. Higher power to detect three-way interactions was observed for RILs derived from optimally allocated distance-based designs than from nested designs or diallel designs. The power and PFP to detect three-way interactions using a nested design with 5000 RILs was for both the 4 QTL and the 12 QTL scenario of a magnitude that seems promising for their identification.
Key Words: Genome sequence, Higher-order epistasis, Maize, Metabolic pathway, RILs
This article has been cited by other articles:
 |
|
 |
|
  M. Maccaferri, M. C. Sanguineti, S. Corneti, J. L. A. Ortega, M. B. Salem, J. Bort, E. DeAmbrogio, L. F. G. del Moral, A. Demontis, A. El-Ahmed, et al. Quantitative Trait Loci for Grain Yield and Adaptation of Durum Wheat (Triticum durum Desf.) Across a Wide Range of Water Availability Genetics, January 1, 2008; 178(1): 489 - 511. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. H. Kebrom and T. P. Brutnell The molecular analysis of the shade avoidance syndrome in the grasses has begun J. Exp. Bot., October 5, 2007; (2007) erm205v1. [Abstract] [Full Text] [PDF]
|
|