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doi:10.1534/genetics.106.066761
A more recent version of this article appeared on June 1, 2007.
REGULAR RESEARCH PAPERS |
31 Flavors of Drosophila Rab Proteins
Jun Zhang 1, Karen L. Schulze 2, P. Robin Hiesinger 3, Kaye Suyama 1, Stream Wang 1, Matthew Fish 1, Melih Acar 2, Roger A. Hoskins 4, Hugo J. Bellen 2 and Matthew P. Scott 1*
1 Stanford University
2 Baylor College of Medicine
3 UT Southwestern Medical Center
4 Lawrence Berkeley National Laboratory
* To whom correspondence should be addressed. E-mail: mscott{at}stanford.edu.
Submitted on October 12, 2006
Revised on November 29, 2006
Accepted on 21 March 2007
Rab proteins are small GTPases that play important roles in transport of vesicle cargo and recruitment, association of motor and other proteins with vesicles, and docking and fusion of vesicles at defined locations. In vertebrates, more than 75 Rab genes have been identified, some of which have been intensively studied for their roles in endosome and synaptic vesicle trafficking. Recent studies of the functions of certain Rab proteins have revealed specific roles in mediating developmental signal transduction. We have begun a systematic genetic study of the 33 Rab genes in Drosophila. Most of the fly proteins are clearly related to specific vertebrate proteins. We report here the creation of a set of transgenic fly lines that allow spatially and temporally regulated expression of Drosophila Rab proteins. We generated fluorescent protein-tagged wild-type, dominant-negative, and constitutively active forms of 31 Drosophila Rab proteins. We describe Drosophila Rab expression patterns during embryogenesis, the subcellular localization of some Rab proteins, and comparisons of the localization of wild-type, dominant-negative, and constitutively active forms of selected Rab proteins. The high evolutionary conservation and low redundancy of Drosophila Rab proteins make these transgenic lines a useful toolkit for investigating Rab functions in vivo.
Key Words: Drosophila, GTPase, Rabs, vesicle trafficking
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