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doi:10.1534/genetics.106.065573
A more recent version of this article appeared on March 1, 2007.
REGULAR RESEARCH PAPERS |
Single loci detection and karyotyping using small target FISH on maize somatic chromosomes
Jonathan C. Lamb 1, Tatiana Danilova 1, Matthew J. Bauer 1, Julie M. Meyer 2, Jennifer J. Holland 1, Michael D. Jensen 1 and James A. Birchler 1*
1 University of Missouri
2 Truman State University
* To whom correspondence should be addressed. E-mail: birchlerj{at}missouri.edu.
Submitted on September 4, 2006
Revised on October 17, 2006
Accepted on 29 December 2006
Combined with a system for identifying each of the chromosomes in a genome, visualizing the location of individual genetic loci by fluorescence in situ hybridization (FISH) would aid in assembling physical and genetic maps. Previously, large genomic clones have been successfully used as FISH probes onto somatic chromosomes but this approach is complicated in species with abundant repetitive elements. In this study, repeat free portions of sequences that were anchored to particular chromosomes including genes, gene clusters, large cDNAs and portions of BACs obtained from public databases were used to label the corresponding physical location using FISH. A collection of probes was assembled that includes at least one marker on each chromosome in the maize complement, allowing a small target karyotyping system to be developed. This set provides the foundation onto which additional loci could be added to strengthen further the ability to perform chromosomal identification in maize and its relatives. The probes were demonstrated to produce signals in several wild relatives of maize including Zea luxurians, Z. diploperennis and Tripsacum dactyloides.
Key Words: FISH, Maize, karyotyping, single gene probe
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