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doi:10.1534/genetics.106.064105
A more recent version of this article appeared on November 1, 2006.
REGULAR RESEARCH PAPERS |
Molecular Assembly of Meiotic Proteins Asy1 and Zyp1 and Pairing Promiscuity in Rye (Secale cereale L.) and Its Synaptic Mutant sy10
Elena I Mikhailova 1, Dylan Phillips 2, Svetlana P Sosnikhina 1, Alexandra V. Lovtsyus 1, Robert Neil Jones 2 and Glyn Jenkins 2*
1 St. Petersburg State University
2 University of Wales Aberystwyth
* To whom correspondence should be addressed. E-mail: gmj{at}aber.ac.uk.
Submitted on July 28, 2006
Revised on August 16, 2006
Accepted on 1 September 2006
Assembly of two orthologous proteins associated with meiotic chromosome axes in Arabidopsis thaliana (Asy1 and Zyp1) was studied immunologically at meiotic prophase of meiosis of wild type rye (Secale cereale) and its synaptic mutant sy10, using antibodies derived from A. thaliana. The temporal and spatial expression of the two proteins were similar in wild type rye, but with one notable difference. Unlike A. thaliana, in which foci of the transverse filament protein Zyp1 appear to linearise commensurately with synapsis, linear tracts of Asy1 and Zyp1 protein form independently at leptotene and early zygotene of rye, and co-align into triple structures resembling synaptonemal complexes (SCs) only at later stages of synapsis. The sy10 mutant used in this study also forms spatially separate linear tracts of Asy1 and Zyp1 proteins at leptotene and early zygotene, and these co-align but do not form regular triple structures at mid-prophase. Electron microscopy of spread axial elements reveals extensive asynapsis with some exchanges of pairing partners. Indiscriminate SCs support non-homologous chiasma formation at metaphase I, as revealed by multi-colour fluorescence in situ hybridisation enabling reliable identification of all the chromosomes of the complement. Scrutiny of chiasmate and non-chiasmate ("sticky") associations of chromosomes at this stage revealed some specificity in the associations of homologous and non-homologous chromosomes. Inferences about the nature of synapsis in this mutant were drawn from such observations.
Key Words: Asy1, Secale cereale, Zyp1, meiosis, sy10
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