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doi:10.1534/genetics.106.063826
A more recent version of this article appeared on May 1, 2007.
REGULAR RESEARCH PAPERS |
Control of sigma virus multiplication by the ref(2)P gene of Drosophila melanogaster. An in vivo study of the PB1 domain of Ref(2)P
Alyssa Carré-Mlouka 1, Sébastien Gaumer 1, Pierre Gay 1, Anne-Marie Petitjean 1, Christine Coulondre 1, Philippe Dru 2, Françoise Bras 3, Sybille Dezélée 3 and Didier Contamine 1*
1 Université Versailles SQY, CNRS
2 Université Paris VI, CNRS
3 CNRS
* To whom correspondence should be addressed. E-mail: didier.contamine{at}uvsq.fr.
Submitted on July 24, 2006
Revised on August 26, 2006
Accepted on 15 February 2007
Ref(2)P has been described as one of Drosophila proteins which interacts with sigma virus cycle. We generated alleles to identify critical residues involved in the restrictive or permissive character of Ref(2)P. We demonstrate that permissive alleles increase the ability of sigma virus to infect Drosophila when compared to null alleles and we confirm that restrictive alleles decrease this capacity. Moreover, we have created alleles unfunctional toward viral cycling while functional for Ref(2)P fly functions. This type of allele had never been observed before and shows that fly- and virus-related activities of Ref(2)P are separable. The viral status of Ref(2)P variants is determined by the amino-terminal PB1 domain polymorphism. In addition, an isolated PB1 domain mimics virus-related functions even if it is fly-unfunctional. The evolutionary tree of Ref(2)P PB1 domain we could build based on natural allele sequences is in aggreement with an evolution of PB1 domain due to sucessive transient selection waves.
Key Words: Drosophila, PB1 domain, Ref(2)P, Sigma virus, Transient selection wave
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