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doi:10.1534/genetics.106.062745
A more recent version of this article appeared on November 1, 2006.
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Molecular-genetic biodiversity in natural population of yeast S. cerevisiae from "Evolution Canyon" Microsatellite polymorphism, ploidy, and controversial sexual status
Tal Katz Ezov 1, Elizabeth Boger-Nagjar 1, Ze'ev Frenkel 2, Ina Katsperovski 1, Stav Kemeny 2, Eviatar Nevo 2, Abraham B. Korol 2 and Yechezkel Kashi 1*
1 Technion
2 University of Haifa
* To whom correspondence should be addressed. E-mail: kashi{at}techunix.technion.ac.il.
Submitted on June 29, 2006
Revised on July 24, 2006
Accepted on 17 August 2006
Yeast S. cerevisiae is a central model organism in eukaryotic cell studies and a major component in many food and biotechnological industrial processes. However, the wide knowledge regarding genetics and molecular biology of S. cerevisiae is based on an extremely narrow range of strains. Studies of natural populations of S. cerevisiae, not associated with human activities or industrial fermentation environments, are very few. We isolated a panel of S. cerevisiae strains from a natural microsite "Evolution Canyon" at Mount Carmel, Israel, and studied their genomic biodiversity. Analysis of 19 microsatellite loci revealed high allelic diversity and variation in ploidy level across the panel, from diploids to tetraploids, confirmed by flow cytometry. No significant differences were found in the level of microsatellite variation between strains derived from the major localities or microniches, whereas strains of different ploidy showed low similarity in allele content. Maximum genetic diversity was observed among diploids, and minimum among triploids. Phylogenetic analysis revealed clonal, rather than sexual, structure of the triploid and tetraploid subpopulations. Viability tests in tetrad analysis also suggest that clonal reproduction may predominate in the polyploid subpopulations.
Key Words: S. cerevisiae, microsatellites, natural isolates, ploidy, polymorphism
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