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doi:10.1534/genetics.106.062604
A more recent version of this article appeared on July 1, 2007.
REGULAR RESEARCH PAPERS |
Functional analysis of maize RAD51 in meiosis and DSBs repair
Jin Li 1, Lisa C. Harper 2, Inna Golubovskaya 2, C. Rachel Wang 2, David Weber 3, Robert B. Meeley 4, John McElver 4, Ben Bowen 4, W. Zacheus Cande 2 and Patrick S. Schnable 1*
1 Iowa State University
2 University of California, Berkeley
3 Illnois State University
4 Pioneer Hi-Bred Intl. Inc.
* To whom correspondence should be addressed. E-mail: schnable{at}iastate.edu.
Submitted on June 29, 2006
Revised on August 23, 2006
Accepted on 8 May 2007
In Saccharomyces cerevisiae, Rad51p plays a central role in homologous recombination and the repair of double-strand breaks (DSBs). Double mutants of the two Zea mays L. (maize) rad51 homologs are viable and develop well under normal conditions, but are male sterile and have substantially reduced seed set. Light microscopic analyses of male meiosis in these plants reveal: reduced homologous pairing, synapsis of non-homologous chromosomes, reduced bivalents at diakinesis, numerous chromosome breaks at anaphase I and that over 33% of quartets carry cells that either lack an organized nucleolus or have two nucleoli. This indicates that RAD51 is required for efficient chromosome pairing and its absence results in non-homologous pairing and synapsis. These phenotypes differ from those of an Arabidopsis rad51 mutant that exhibits completely disrupted chromosome pairing and synapsis during meiosis. Unexpectedly, surviving female gametes produced by maize rad51 double mutants are euploid and exhibit near-normal rates of meiotic crossovers. The finding that maize rad51 double mutant embryos are extremely susceptible to radiation-induced DSBs demonstrates a conserved role for RAD51 in the repair of mitotic DSBs in plants, vertebrates and yeast.
Key Words: RAD51, chromosome pairing, double-strand breaks, meiotic crossovers, synapsis
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  J. Li, T.-J. Wen, and P. S. Schnable Role of RAD51 in the Repair of MuDR-Induced Double-Strand Breaks in Maize (Zea mays L.) Genetics, January 1, 2008; 178(1): 57 - 66. [Abstract] [Full Text] [PDF]
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