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doi:10.1534/genetics.106.060699
A more recent version of this article appeared on August 1, 2006.
REGULAR RESEARCH PAPERS |
Cis-transcriptional variation in maize inbred lines B73 and Mo17 lead to additive expression patterns in the F1 hybrid
Robert M Stupar 1 and Nathan M Springer 1*
1 University of Minnesota
* To whom correspondence should be addressed. E-mail: springer{at}umn.edu.
Submitted on May 12, 2006
Revised on May 12, 2006
Accepted on 12 May 2006
Microarray analysis of gene expression patterns in immature ear, seedling and embryo tissues from the maize inbred lines B73 and Mo17 identified numerous genes with variable expression. Some genes had detectable expression in only one of the two inbreds; most of these genes were detected in the genomic DNA of both inbreds, indicating that the expression differences are likely caused by differential regulation rather than differences in gene content. Gene expression was also monitored in the reciprocal F1 hybrids B73XMo17 and Mo17XB73. The reciprocal F1 hybrid lines did not display parental affects on gene expression levels. Approximately 80% of the differentially expressed genes displayed additive expression patterns in the hybrids relative to the inbred parents. The ~20% of genes that display non-additive expression patterns tend to be expressed at levels within the parental range, with minimal evidence for novel expression levels above the high-parent or below the low-parent. Analysis of allele-specific expression patterns in the hybrid suggested that intraspecific variation in gene expression levels is largely attributable to cis-regulatory variation in maize. Collectively, our data suggest that allelic cis-regulatory variation between B73 and Mo17 dictates maintenance of inbred allelic expression levels in the F1 hybrid, resulting in additive expression patterns.
Key Words: allele-specific expression, cis-regulatory variation, gene expression
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