Genetics. Published Articles Ahead of Print: December 18, 2006, Copyright © 2006
doi:10.1534/genetics.106.059972


A more recent version of this article appeared on February 1, 2007.

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A Microsatellite Linkage Map of Barramundi, Lates calcarifer

C.M. Wang 1, Z.Y. Zhu 1, L.C. Lo 1, F. Feng 1, G. Lin 1, W.T. Yang 1, J. Li 1 and G.H. Yue 1*

1 Temasek Life Sciences Laboratory

* To whom correspondence should be addressed. E-mail: genhua{at}tll.org.sg.

Submitted on April 26, 2006
Revised on September 1, 2006
Accepted on 1 December 2006


Abstract

Barramundi (Lates calcarifer) is an important farmed marine foodfish species. Its compact genome (~700 Mb) is among the smallest genomes of food fish species. We established a first generation genetic linkage map of Barramundi with a mapping panel containing three parents (two males and one female) and 93 progenies. Two hundred and forty microsatellite markers were mapped into 24 linkage groups. Among these markers, 10 were located in ESTs and known genes. The total lengths of the female and male maps were 873.8 cM and 414.5 cM with an average marker spacing of 6.20 cM and 4.70 cM respectively. Comparing the flanking sequences of the 240 Barramundi microsatellites with the assembled whole genome sequences of Tetraodon nigrovidiris revealed 55 homologous sequences located in 19 of the 21 chromosomes of Tetraodon nigrovidiris. The map will not only enable the mapping of quantitative trait loci, but also provide new resources for understanding the evolution of fish genomes.

Key Words: Barramundi, Comparative genomics, Linkage map, Microsatellites




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