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doi:10.1534/genetics.106.058933
A more recent version of this article appeared on December 1, 2006.
REGULAR RESEARCH PAPERS |
Centromere-Proximal Crossovers Are Associated with Precocious Separation of Sister Chromatids During Meiosis in Saccharomyces cerevisiae
Beth M Rockmill 1, Karen Voelkel-Meiman 1 and G. Shirleen Roeder 1*
1 Howard Hughes Medical Institute
* To whom correspondence should be addressed. E-mail: shirleen.roeder{at}yale.edu.
Submitted on April 4, 2006
Revised on May 10, 2006
Accepted on 24 September 2006
ABSTRACT In most organisms, meiotic chromosome segregation is dependent on crossovers (COs), which enable pairs of homologous chromosomes to segregate to opposite poles at meiosis I. In mammals, the majority of meiotic chromosome segregation errors result from a lack of COs between homologs. Observations in H. sapiens and D. melanogaster have revealed a second class of exceptional events in which a CO occurred near the centromere of the missegregated chromosome. We show that in wild-type strains of S. cerevisiae, most spore inviability is due to precocious separation of sister chromatids (PSSC) and that PSSC is often associated with centromere-proximal crossing over. COs, rather than nonreciprocal recombination events (NCOs), are preferentially associated with missegregation. Strains mutant for the RecQ homolog, SGS1, display reduced spore viability and increased crossing over. Much of the spore inviability in sgs1 results from PSSC, and these events are often associated with centromere-proximal COs, just as in wild type. When crossing over is reduced in sgs1 by the introduction of a non-null allele of SPO11, spore viability is improved, suggesting that the increased PSSC is due to increased crossing over. We present a model for PSSC in which a centromere-proximal CO promotes local loss of sister-chromatid cohesion.
Key Words: SGS1, aneuploidy, meiosis, recombination, yeast
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