- THIS ARTICLE
- Full Text (Rapid PDF)
- Data Supplement
-
All Versions of this Article:
genetics.106.058818v1
174/1/363 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Mateos, M.
- Articles by Moran, N. A
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Mateos, M.
- Articles by Moran, N. A
doi:10.1534/genetics.106.058818
A more recent version of this article appeared on September 1, 2006.
REGULAR RESEARCH PAPERS |
Heritable Endosymbionts of Drosophila
Mariana Mateos 1*, Sergio J. Castrezana 2, Becky J. Nankivell 2, Anne M Estes 2, Therese A Markow 2 and Nancy A Moran 2
1 The University of Texas at El Paso
2 University of Arizona
* To whom correspondence should be addressed. E-mail: mmateos{at}utep.edu.
Submitted on April 12, 2006
Revised on May 18, 2006
Accepted on 4 June 2006
Although heritable microorganisms are increasingly recognized as widespread in insects, no systematic screens for such symbionts have been conducted in Drosophila species (the primary insect genetic models for studies of evolution, development, and innate immunity). Previous efforts screened relatively few Drosophila lineages, mainly for Wolbachia. We conducted an extensive survey of potentially heritable endosymbionts from any bacterial lineage via PCR screens of mature ovaries in 181 recently collected fly strains representing 35 species from 11 species groups. Due to our fly sampling methods however, we are likely to have missed fly strains infected with sex ratio distorting endosymbionts. Only Wolbachia and Spiroplasma, both widespread in insects, were confirmed as symbionts. These findings indicate that in contrast to some other insect groups, other heritable symbionts are uncommon in Drosophila species, possibly reflecting a robust innate immune response that eliminates many bacteria. A more extensive survey targeted these two symbiont types through diagnostic PCR in 1225 strains representing 225 species from 32 species groups. Of these, 19 species were infected by Wolbachia while only three species had Spiroplasma. Several new strains of Wolbachia and Spiroplasma were discovered, including ones divergent from any reported to date. The phylogenetic distribution of Wolbachia and Spiroplasma in Drosophila is discussed.
Key Words: 16S rRNA, Drosophilidae, Spiroplasma, Wolbachia, wsp
This article has been cited by other articles:
 |
|
 |
|
  L. M. Matzkin The Molecular Basis of Host Adaptation in Cactophilic Drosophila: Molecular Evolution of a Glutathione S-Transferase Gene (GstD1) in Drosophila mojavensis Genetics, February 1, 2008; 178(2): 1073 - 1083. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Corby-Harris, A. C. Pontaroli, L. J. Shimkets, J. L. Bennetzen, K. E. Habel, and D. E. L. Promislow Geographical Distribution and Diversity of Bacteria Associated with Natural Populations of Drosophila melanogaster Appl. Envir. Microbiol., June 1, 2007; 73(11): 3470 - 3479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. R. Cox and M. S. Gilmore Native Microbial Colonization of Drosophila melanogaster and Its Use as a Model of Enterococcus faecalis Pathogenesis Infect. Immun., April 1, 2007; 75(4): 1565 - 1576. [Abstract] [Full Text] [PDF]
|
|