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doi:10.1534/genetics.106.058768
A more recent version of this article appeared on August 1, 2006.
REGULAR RESEARCH PAPERS |
S. cerevisiae Mer2, Mei4 and Rec114 Form a Complex Required for Meiotic Double-Strand Break Formation
Jing Li 1, Gillian W. Hooker 1 and G. Shirleen Roeder 2*
1 Yale University
2 Howard Hughes Medical Institute, Yale University
* To whom correspondence should be addressed. E-mail: shirleen.roeder{at}yale.edu.
Submitted on March 30, 2006
Revised on May 6, 2006
Accepted on 6 June 2006
In budding yeast, at least ten proteins are required for formation of the double-strand breaks (DSBs) that initiate meiotic recombination. Spo11 is the enzyme responsible for cleaving DNA and is found in a complex that also contains Ski8, Rec102 and Rec104. The Mre11/Rad50/Xrs2 complex is required for both DSB formation and DSB processing. In this paper, we investigate the functions of the remaining three proteins - Mer2, Mei4 and Rec114 - with particular emphasis on Mer2. The Mer2 protein is present in vegetative cells, but it increases in abundance and becomes phosphorylated specifically during meiotic prophase. Mer2 localizes to distinct foci on meiotic chromosomes, with foci maximally abundant prior to the formation of synaptonemal complex. If DSB formation is blocked (e.g., by a spo11 mutation), dephosphorylation of Mer2 and its dissociation from chromosomes are delayed. We have also found that the Mei4 and Rec114 proteins localize to foci on chromosomes, and these foci partially colocalize with each other and with Mer2. Furthermore, the three proteins coimmunoprecipitate. Mer2 does not show significant colocalization with Mre11 or Rec102, and Mer2 does not coimmunoprecipitate with Rec102. We propose that Mer2, Mei4 and Rec114 form a distinct complex required for DSB formation.
Key Words: S. cerevisiae, Double-Strand Break, Meiosis, Mer2, Recombination
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