- THIS ARTICLE
- Full Text (Rapid PDF)
- Data Supplement
-
All Versions of this Article:
genetics.106.058156v1
174/1/179 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Federico, M. L
- Articles by Kaeppler, H. F
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Federico, M. L
- Articles by Kaeppler, H. F
doi:10.1534/genetics.106.058156
A more recent version of this article appeared on September 1, 2006.
REGULAR RESEARCH PAPERS |
Spatial and Temporal Divergence of Expression in Duplicated Barley Germin-like Protein-encoding Genes
Maria L Federico 1, Federico L Iñiguez-Luy 1, Ronald W Skadsen 2 and Heidi F Kaeppler 1*
1 University of Wisconsin-Madison
2 USDA-ARS-CCRU
* To whom correspondence should be addressed. E-mail: hfkaeppl{at}wisc.edu.
Submitted on March 14, 2006
Revised on April 25, 2006
Accepted on 28 May 2006
Subfunctionalization is the process by which a pair of duplicated genes, or paralogs, experiences a reduction of individual expression patterns or function while still reproducing the complete expression pattern and function of the ancestral gene. Two germin-like protein (GLP) encoding genes, GerB and GerF, are paralogs which belong to a small gene family in barley (Hordeum vulgare). Both genes share high nucleotide sequence similarity in coding and non-coding regions, and encode identical apoplastic proteins. The use of RNA gel blots, coupled with single-stranded conformation polymorphism (SSCP) analysis of RT-PCR products, elucidated the developmental and tissue-specific expression patterns of each gene. Individual expression patterns provided evidence of both overlapping redundancy and early subfunctionalization. GerB is predominantly expressed in developing shoots, while GerF is predominantly expressed in seedling roots, developing spikes and pericarp/testa. GerF promoter deletion studies located a region (-356/-97) responsible for high promoter activity and showed the ability of GerB and GerF upstream regions to drive gfp expression in coleoptiles, epicarps, and lemma/palea of developing spikes. The observed expression patterns are consistent with proposed roles in plant development and defense mechanisms for this gene family. These roles may explain why redundancy has been selectively maintained in this duplicate gene pair.
Key Words: duplicated genes, gemin like proteins, paralogs, subfunctionalization, tissue specific promoters
This article has been cited by other articles:
 |
|
 |
|
  K. L. Adams Evolution of Duplicate Gene Expression in Polyploid and Hybrid Plants J. Hered., March 1, 2007; 98(2): 136 - 141. [Abstract] [Full Text] [PDF]
|
|