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doi:10.1534/genetics.106.057554
A more recent version of this article appeared on June 1, 2006.
REGULAR RESEARCH PAPERS |
Numerous and rapid non-stochastic modifications of gene products in newly synthesized Brassica napus allotetraploids
Warren Albertin 1*, Thierry Balliau 1, Philippe Brabant 1, Anne-Marie Chevre 2, Fréderique Eber 2, Christian Malosse 3 and Hervé Thiellement 1
1 UMR de Génétique Végétale
2 UMR ENSAR-INRA
3 de Phytopharmacie et Médiateurs Chimiques
* To whom correspondence should be addressed. E-mail: albertin{at}moulon.inra.fr.
Submitted on February 23, 2006
Revised on April 6, 2006
Accepted on 6 April 2006
Polyploidization is a widespread process that results in the merger of two or more genomes in a common nucleus. To investigate modifications of gene expression occurring during allopolyploid formation, the Brassica napus allotetraploid model was chosen. Large-scale analyses of the proteome were conducted on two organs, the stem and root, so that more than 1,600 polypeptides were screened. Comparative proteomics of synthetic B. napus and its homozygous diploid progenitors B. rapa and B. oleracea showed that very few proteins disappeared or appeared in the amphiploids (<1%), but a strikingly high number (25-38%) of polypeptides displayed quantitative non-additive pattern. Non-stochastic gene expression re-patterning was found since 99% of the detected variations were reproducible in four independently created amphiploids. More than 60% of proteins displayed a non-additive pattern closer to the paternal parent B. rapa. Interspecific hybridization triggered the majority of the deviations (89%), whereas very few variations (~3%) were associated with genome doubling and more significant alterations arose from selfing (~9%). Some non-additive proteins behaved similarly in both organs, while others exhibited contrasted behavior, showing rapid organ-specific regulation. Brassica napus formation was therefore correlated with immediate and directed non-additive changes in gene expression, suggesting that the early steps of allopolyploidization repatterning are controlled by non-stochastic mechanisms.
Key Words: allopolyploid, polyploidy, proteomics, two-dimensional electrophoresis
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