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doi:10.1534/genetics.106.056721
A more recent version of this article appeared on July 1, 2006.
REGULAR RESEARCH PAPERS |
The molecular identities of the Caenorhabditis elegans intraflagellar transport genes dyf-6, daf-10, and osm-1
Leslie R. Bell 1*, Steven Stone 2, John Yochem 1, Jocelyn E. Shaw 1 and Robert K. Herman 1
1 University of Minnesota
2 Myriad Genetics, Inc.
* To whom correspondence should be addressed. E-mail: bellx035{at}umn.edu.
Submitted on February 3, 2006
Revised on March 15, 2006
Accepted on 28 April 2006
The Caenorhabditis elegans genes dyf-6, daf-10, and osm-1 are among the set of genes that affect chemotaxis and the ability of certain sensory neurons to take up fluorescent dyes from the environment. Some genes in this category are known to be required for intraflagellar transport (IFT), which is the bidirectional movement of raft-like particles along the axonemes of cilia and flagella. The cloning of dyf-6, daf-10, and osm-1 are described here. The daf-10 and osm-1 gene products resemble each other and contain WD and WAA repeats. DYF-6, the product of a complex locus, lacks known motifs, but orthologs are present in flies and mammals. Phenotypic analysis of dyf-6 mutants expressing an OSM-6::GFP reporter indicates that the cilia of the amphid and phasmid dendritic endings are foreshortened. Consistent with genetic mosaic analysis, which indicates that dyf-6 functions in neurons of the amphid sensilla, DYF-6::GFP is expressed in amphid and phasmid neurons. Movement of DYF-6::GFP along the ciliated endings of the neurons indicates that DYF-6 is a component of IFT particles. IFT can also be observed in dauer larvae.
Key Words: IFT, amphids, cilia, dauer larvae, dye-filling
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