- THIS ARTICLE
- Full Text (Rapid PDF)
-
All Versions of this Article:
genetics.105.053801v1
173/1/151 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Yamamoto, K.
- Articles by Mita, K.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Yamamoto, K.
- Articles by Mita, K.
doi:10.1534/genetics.105.053801
A more recent version of this article appeared on May 1, 2006.
REGULAR RESEARCH PAPERS |
Construction of a Single Nucleotide Polymorphism Linkage Map for the Silkworm, Bombyx mori, Based on BAC End-Sequences
Kimiko Yamamoto 1*, Junko Narukawa 1, Keiko Kadono-Okuda 1, Junko Nohata 1, Motoe Sasanuma 1, Yoshitaka Suetsugu 1, Yutaka Banno 2, Hiroshi Fujii 2, Marian R Goldsmith 3 and Kazuei Mita 1
1 National Institute of Agrobiological Sciences
2 Kyushu University
3 University of Rhode Island
* To whom correspondence should be addressed. E-mail: kiya{at}nias.affrc.go.jp.
Submitted on November 22, 2005
Revised on December 26, 2005
Accepted on 6 March 2006
We have developed a linkage map for the silkworm Bombyx mori based on single nucleotide polymorphisms (SNPs) between strains p50T and C108T initially found on regions corresponding to the end sequences of bacterial artificial chromosome (BAC) clones. Using 190 segregants from a backcross of a p50T female x an F1 (p50T x C108T) male we analyzed segregation patterns of 534 SNPs between p50T and C108T detected among 3,840 PCR amplicons, each associated with a p50T BAC end-sequence. This enabled us to construct a linkage map composed of 534 SNP markers spanning 1,305 cM in total length distributed over the expected 28 linkage groups. Of the 534 BACs whose ends harbored the SNPs used to construct the linkage map, 89 were associated with 107 different ESTs. Since each of the SNP markers is directly linked to a specific genomic BAC clone and to whole genome sequence data, and some of them are also linked to EST data, the SNP linkage map will be a powerful tool for investigating silkworm genome properties, mutation mapping, and map-based cloning of genes of industrial and agricultural interest.
Key Words: Bacterial artificial chromosome (BAC), Bombyx mori, Linkage map, Silkworm, Single nucleotide polymorphism (SNP)
This article has been cited by other articles:
 |
|
 |
|
  K. Ito, K. Kidokoro, H. Sezutsu, J. Nohata, K. Yamamoto, I. Kobayashi, K. Uchino, A. Kalyebi, R. Eguchi, W. Hara, et al. Deletion of a gene encoding an amino acid transporter in the midgut membrane causes resistance to a Bombyx parvo-like virus PNAS, May 27, 2008; 105(21): 7523 - 7527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Papanicolaou, S. Gebauer-Jung, M. L. Blaxter, W. Owen McMillan, and C. D. Jiggins ButterflyBase: a platform for lepidopteran genomics Nucleic Acids Res., January 11, 2008; 36(suppl_1): D582 - D587. [Abstract] [Full Text] [PDF]
|
|