- THIS ARTICLE
- Full Text (Rapid PDF)
-
All Versions of this Article:
genetics.105.052415v1
172/4/2201 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Malagon, F.
- Articles by Strathern, J. N.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Malagon, F.
- Articles by Strathern, J. N.
doi:10.1534/genetics.105.052415
A more recent version of this article appeared on April 1, 2006.
REGULAR RESEARCH PAPERS |
Mutations in the Saccharomyces cerevisiae RPB1 Gene Conferring Hypersensitivity to 6-Azauracyl
Francisco Malagon 1, Maria L. Kireeva 1, Brenda K. Shafer 1, Lucyna Lubkowska 1, Mikhail Kashlev 1 and Jeffrey N. Strathern 1*
1 NCI at Frederick (NIH)
* To whom correspondence should be addressed. E-mail: strather{at}ncifcrf.gov.
Submitted on October 14, 2005
Revised on November 28, 2005
Accepted on 30 January 2006
RNA polymerase II (RNAPII) in eukaryotic cells drives transcription of most messenger RNAs. RNAPII core enzyme is composed of twelve polypeptides where Rpb1 is the largest subunit. In order to further understand the mechanisms of RNAPII transcription, we isolated and characterized novel point mutants of RPB1 that are sensitive to the nucleotide depleting drug 6-azauracyl (6AU). In this work we have re-isolated the rpo21-24/rpb1-E1230K allele which reduces the interaction of RNAPII with TFIIS, and identified five new point mutations in RPB1 that cause hypersensitivity to 6AU. The novel mutants affect highly conserved residues of Rpb1 and have differential genetic and biochemical effects. Three of the mutations affect the "lid" and "rudder," two small loops suggested by structural studies to play a central role in the separation of the RNA-DNA hybrids. Most interestingly, two mutations affecting the catalytic center (rpb1-N488D) and the homology box G (rpb1-E1103G) have strong opposite effects on the intrinsic in vitro polymerization rate of RNAPII. Moreover, the synthetic interactions of those two last mutants with soh1, spt4 and dst1 suggest differential in vivo effects.
Key Words: 6-azauracyl, RNA elongation, RNA polymerase II, RPB1, lid and rudder
This article has been cited by other articles:
 |
|
 |
|
  J. Andrecka, B. Treutlein, M. A. I. Arcusa, A. Muschielok, R. Lewis, A. C. M. Cheung, P. Cramer, and J. Michaelis Nano positioning system reveals the course of upstream and nontemplate DNA within the RNA polymerase II elongation complex Nucleic Acids Res., September 1, 2009; 37(17): 5803 - 5809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mosrin-Huaman, R. Honorine, and A. R. Rahmouni Expression of Bacterial Rho Factor in Yeast Identifies New Factors Involved in the Functional Interplay between Transcription and mRNP Biogenesis Mol. Cell. Biol., August 1, 2009; 29(15): 4033 - 4044. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Walmacq, M. L. Kireeva, J. Irvin, Y. Nedialkov, L. Lubkowska, F. Malagon, J. N. Strathern, and M. Kashlev Rpb9 Subunit Controls Transcription Fidelity by Delaying NTP Sequestration in RNA Polymerase II J. Biol. Chem., July 17, 2009; 284(29): 19601 - 19612. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. L. Kireeva and M. Kashlev From the Cover: Mechanism of sequence-specific pausing of bacterial RNA polymerase PNAS, June 2, 2009; 106(22): 8900 - 8905. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Malagon and T. H. Jensen The T Body, a New Cytoplasmic RNA Granule in Saccharomyces cerevisiae Mol. Cell. Biol., October 1, 2008; 28(19): 6022 - 6032. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. A. Gibney, T. Fries, S. M. Bailer, and K. A. Morano Rtr1 Is the Saccharomyces cerevisiae Homolog of a Novel Family of RNA Polymerase II-Binding Proteins Eukaryot. Cell, June 1, 2008; 7(6): 938 - 948. [Abstract] [Full Text] [PDF]
|
|
|