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doi:10.1534/genetics.105.050815
A more recent version of this article appeared on February 1, 2006.
REGULAR RESEARCH PAPERS |
Genetics and Molecular Characterization of the I Locus of Phaseolus vulgaris
C. Eduardo Vallejos 1*, Gustavo Astua-Monge 1, Valerie Jones 1, Tammy R Plyler 1, Ney S Sakiyama 2 and Sally A Mackenzie 3
1 University of Florida
2 Universidade Federal de Vicosa
3 University of Nebraska
* To whom correspondence should be addressed. E-mail: vallejos{at}ufl.edu.
Submitted on September 7, 2005
Revised on October 10, 2005
Accepted on 24 October 2005
The I locus of the common bean, Phaseolus vulgaris, controls the development of four different phenotypes in response to inoculation with Bean common mosaic virus, Bean common mosaic necrosis virus, several other related potyviruses and one comovirus. We have generated a high resolution linkage map around this locus, and have aligned it with a physical map constructed with BAC clones. These clones were obtained from a library of the cv. 'Sprite' which carries the dominant allele at the I locus. We have identified a large cluster of TIR-NBS-LRR sequences associated within this locus which extends over a distance in excess of 425 Kb. Bean cultivars from the Andean or Mesoamerican gene pool that contain the dominant allele share the same haplotypes as revealed by gel blot hybridizations with a TIR probe. In contrast, beans with a recessive allele display simpler and variable haplotypes. A survey of wild accessions from Argentina to Mexico showed that this multigene family has expanded significantly during evolution and domestication. RNA gel blot analysis indicated that the TIR family of genes plays a role in the response to inoculations with BCMV or BCMNV.
Key Words: Resistance to potyviruses, TIR-NBS-LRR sequences, suppression of recombination, tandem and ectopic duplications
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