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doi:10.1534/genetics.105.050658
A more recent version of this article appeared on July 1, 2006.
REGULAR RESEARCH PAPERS |
Analysis of close stable homolog juxtaposition during meiosis in mutants of Saccharomyces cerevisiae
Doris Y. Lui 1, Tamara L. Peoples-Holst 1, Joshua Chang Mell 1, Hsin-Yen Wu 1, Eric Dean 2 and Sean M. Burgess 1*
1 University of California, Davis
2 University of California, San Francisco
* To whom correspondence should be addressed. E-mail: smburgess{at}ucdavis.edu.
Submitted on September 15, 2005
Revised on October 4, 2005
Accepted on 29 April 2006
A unique aspect of meiosis is the segregation of homologous chromosomes at the meiosis I division. The pairing of homologous chromosomes is a critical aspect of meiotic prophase I that aids proper disjunction at anaphase I. We have used a site-specific recombination assay in Saccharomyces cerevisiae to examine allelic interaction levels during meiosis in a series of mutants defective in chromatin structure, recombination, or intracellular movement . Red1, a component of the chromosome axis, and Mnd1, a chromosome binding protein that facilitates interhomolog interaction, are critical for achieving high levels of allelic interaction. Homologous recombination factors (Sae2, Rdh54, Rad54, Rad55, Rad51, Sgs1) aid in varying degrees in promoting allelic interactions, while the Srs2 helicase appears to play no appreciable role. Ris1 (a SWI2/SNF2 related protein) and Dot1 (a histone methyltransferase) appear to play minor roles. Surprisingly, factors involved in microtubule-mediated intra-cellular movement (Tub3, Dhc1 and Mlp2) appear to play no appreciable role in homolog juxtaposition, unlike their counterparts in fission yeast. Taken together these results support the notion that meiotic recombination plays a major role in the high levels of homolog interaction observed during budding yeast meiosis.
Key Words: budding yeast, chromosome, homolog pairing, meiosis, recombination
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