Genetics. Published Articles Ahead of Print: December 15, 2005, Copyright © 2005
doi:10.1534/genetics.105.050617


A more recent version of this article appeared on March 1, 2006.

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New Arabidopsis recombinant inbred line populations genotyped using SNPWaveTM and their use for mapping flowering time QTLs

Mohamed E El-Lithy 1, Leonie Bentsink 2, Corrie J Hanhart 1, Gerda Ruys 1, Daniela Rovito 1, Jose L.M. Broekhof 3, Hein J.A. van der Poel 3, Michiel J.T. van Eijk 3, Dick Vreugdenhil 1 and Maarten Koornneef 1*

1 Wageningen University
2 Utrecht University
3 Keygene NV

* To whom correspondence should be addressed. E-mail: maarten.koornneef{at}wur.nl.

Submitted on September 6, 2005
Revised on October 30, 2005
Accepted on 29 November 2005


Abstract

The SNPWaveTM marker system, based on SNPs between the reference accessions Colombia-0 and Landsberg erecta (Ler), was used to distinguish a set of 92 Arabidopsis accessions from various parts of the world. In addition we used these markers to genotype three new recombinant inbred line populations for Arabidopsis, having Ler as a common parent that was crossed with the accessions Antwerp-1, Kashmir-2 and Kondara. The benefit of using multiple populations that contain many similar markers and the fact that all markers are linked to the physical map of Arabidopsis facilitates the quantitative comparison of maps. Flowering time variation was analyzed in the three recombinant inbred line populations. Per population, four to eight quantitative trait loci (QTLs) were detected. The comparison of the QTL positions related to the physical map allowed the estimate of 12 different QTLs segregating for flowering time for which Ler has a different allele from one, two or three of the other accessions.

Key Words: Arabidopsis, Flowering time, QTL,, SNP, SNPWave




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