- THIS ARTICLE
- Full Text (Rapid PDF)
- Supplemental Data
-
All Versions of this Article:
genetics.105.050468v1
172/3/1511 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Vyas, M.
- Articles by Kasbekar, D. P.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Vyas, M.
- Articles by Kasbekar, D. P.
doi:10.1534/genetics.105.050468
A more recent version of this article appeared on March 1, 2006.
REGULAR RESEARCH PAPERS |
Chromosome segment duplications in Neurospora crassa and their effects on repeat-induced point mutation (RIP) and meiotic silencing by unpaired DNA
Meenal Vyas 1, C. Ravindran 1 and Durgadas P. Kasbekar 1*
1 Centre for Cellular and Molecular Biology
* To whom correspondence should be addressed. E-mail: kas{at}ccmb.res.in.
Submitted on September 2, 2005
Revised on November 10, 2005
Accepted on 5 December 2005
The size and extent of four Neurospora crassa duplications, Dp(AR17), Dp(IBj5), Dp(OY329) and Dp(B362i) was determined by testing the coverage of RFLP markers. The first three duplications were all > ~350 kb and have been shown in earlier studies to act as dominant suppressors of repeat-induced point mutation (RIP) in gene-sized duplications, possibly via titration of the RIP machinery. Dp(B362i), which is only about 117 kb long, failed to suppress RIP. RIP suppression in gene-sized duplications by large duplications was demonstrated using another test gene, dow, and supposedly applies generally. Crosses homozygous for Dp(AR17) or Dp(IBj5) were as barren as heterozygous crosses. Barrenness of the heterozygous but not the homozygous crosses was suppressible by Sad-1, a semi-dominant suppressor of RNAi-dependent meiotic silencing by unpaired DNA. A model is proposed in which large duplications recessively suppress semi-dominant Sad-1 mutations. The wild-isolated Sugartown strain is hypothesized to contain a duplication that confers not only dominant suppression of RIP but also a barren phenotype, which is linked (9%) to supercontig 7.118 in LG VII.
Key Words: dominant suppression of RIP, duplication homozygous crosses, suppression of Sad-1
This article has been cited by other articles:
 |
|
 |
|
  R. Tamuli and D. P. Kasbekar Dominant Suppression of Repeat-Induced Point Mutation in Neurospora crassa by a Variant Catalytic Subunit of DNA Polymerase-{zeta} Genetics, March 1, 2008; 178(3): 1169 - 1176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Lee and C.-t. Wu Enhancer-Promoter Communication at the yellow Gene of Drosophila melanogaster: Diverse Promoters Participate in and Regulate trans Interactions Genetics, December 1, 2006; 174(4): 1867 - 1880. [Abstract] [Full Text] [PDF]
|
|