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genetics.105.048231v1
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doi:10.1534/genetics.105.048231
A more recent version of this article appeared on April 1, 2006.
REGULAR RESEARCH PAPERS |
Snf1-dependent and Snf1-independent constitutive pathways of ADH2 expression in Saccharomyces cerevisiae
Valentina Voronkova 1, Nataly Kacherovsky 1, Christine Tachibana 1, Diana Yu 1 and Elton T. Young 1*
1 University of Washington
* To whom correspondence should be addressed. E-mail: ety{at}u.washington.edu.
Submitted on July 13, 2005
Revised on August 15, 2005
Accepted on 3 January 2006
The transcription factor Adr1 directly activates the expression of genes encoding enzymes in numerous pathways that are up-regulated after the exhaustion of glucose in the yeast Saccharomyces cerevisiae. ADH2, encoding the alcohol dehydrogenase isozyme required for ethanol oxidation, is a highly glucose repressed, Adr1-dependent gene. Using a genetic screen we isolated over one-hundred mutants in twelve complementation groups that exhibit ADR1-dependent constitutive ADH2 expression on glucose. Temperature sensitive alleles are present among the new constitutive mutants, indicating that essential genes play a role in ADH2 repression. Among the genes we cloned are MOT1, encoding a repressor that inhibits TBP binding to the promoter, thus linking glucose repression with TBP access to chromatin. Two genes encoding proteins involved in vacuolar function, FAB1 and VPS35, and CDC10, encoding a non-essential septin were also uncovered in the search, suggesting that vacuolar function and the cytoskeleton have previously unknown roles in regulating gene expression. Constitutive activation of ADH2 expression by Adr1 is SNF1-dependent in a strain with a defective MOT1 gene whereas deletion of SNF1 did not affect constitutive ADH2 expression in the mutants affecting vacuolar or septin function. Thus, the mutant search revealed previously unknown Snf1-dependent and independent pathways of ADH2 expression.
Key Words: MOT1, glucose repression, phosphoinositol kinase, septins, transcription regulation
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