- THIS ARTICLE
- Full Text (Rapid PDF)
-
All Versions of this Article:
genetics.105.048058v1
172/4/2211 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by KOSZUL, R.
- Articles by Fischer, G.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by KOSZUL, R.
- Articles by Fischer, G.
doi:10.1534/genetics.105.048058
A more recent version of this article appeared on April 1, 2006.
REGULAR RESEARCH PAPERS |
Stability of large segmental duplications in the yeast genome
Romain KOSZUL 1, Bernard Dujon 2 and Gilles Fischer 2*
1 Harvard University
2 Institut Pasteur
* To whom correspondence should be addressed. E-mail: fischer{at}pasteur.fr.
Submitted on July 12, 2005
Revised on September 9, 2005
Accepted on 6 February 2006
The high level of gene redundancy that characterizes eukaryotic genomes results in part from segmental duplications. Spontaneous duplications of large chromosomal segments have been experimentally demonstrated in yeast. However, the dynamics of inheritance of such structures and their eventual fixation in populations remain largely unsolved. We analyzed the stability of a vast panel of large segmental duplications in Saccharomyces cerevisiae (from 41 kb for the smallest to 268 kb for the largest). We monitored the stability of three different types of interchromosomal duplications as well as that of three intrachromosomal direct tandem duplications. In the absence of any selective advantage associated with the presence of the duplication, we show that a duplicated segment internally translocated within a natural chromosome is stably inherited both mitotically and meiotically. By contrast, large duplications carried by a supernumerary chromosome are highly unstable. Duplications translocated into subtelomeric regions are lost at variable rates depending on the location of the insertion sites. Direct tandem duplications are lost by unequal crossing over, both mitotically and meiotically, at a frequency proportional to their sizes. These results show that most of the duplicated structures present an intrinsic level of instability. However, translocation within another chromosome significantly stabilizes a duplicated segment increasing its chance to get fixed in a population even in the absence of any immediate selective advantage conferred by the duplicated genes.
Key Words: chromosomal rearrangement, experimental evolution, genome evolution and stability, segmental duplication, translocation
This article has been cited by other articles:
 |
|
 |
|
  G.-F. Richard, A. Kerrest, and B. Dujon Comparative Genomics and Molecular Dynamics of DNA Repeats in Eukaryotes Microbiol. Mol. Biol. Rev., December 1, 2008; 72(4): 686 - 727. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Wei, R. E. Davis, R. Jomantiene, and Y. Zhao Ancient, recurrent phage attacks and recombination shaped dynamic sequence-variable mosaics at the root of phytoplasma genome evolution PNAS, August 19, 2008; 105(33): 11827 - 11832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Brochet, E. Couve, M. Zouine, C. Poyart, and P. Glaser A Naturally Occurring Gene Amplification Leading to Sulfonamide and Trimethoprim Resistance in Streptococcus agalactiae J. Bacteriol., January 15, 2008; 190(2): 672 - 680. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Marcello and J. D. Barry Analysis of the VSG gene silent archive in Trypanosoma brucei reveals that mosaic gene expression is prominent in antigenic variation and is favored by archive substructure Genome Res., September 1, 2007; 17(9): 1344 - 1352. [Abstract] [Full Text] [PDF]
|
|
|