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doi:10.1534/genetics.105.047662
A more recent version of this article appeared on January 1, 2006.
NOTE |
Eliminating gene conversion improves high-throughput genetics in Saccharomyces cerevisiae
Jewel A Daniel 1, Jiyoun Y. Yoo 1, Blaine T. Bettinger 2, David C. Amberg 2 and Daniel J. Burke 1*
1 University of Virginia Medical Center
2 Upstate Medical Center
* To whom correspondence should be addressed. E-mail: dburke{at}virginia.edu.
Submitted on July 1, 2005
Revised on August 4, 2005
Accepted on 10 September 2005
Synthetic genetic analysis was improved by eliminating leaky expression of the HIS3 reporter and gene conversion between the HIS3 reporter and his3
1. Leaky expression was eliminated using 3-amino-triazole and gene conversion was eliminated by using the Schizzosaccharomyces pombe his5+ gene resulting in a 5-10 fold improvement in the efficiency of SGA.
Key Words: spindle checkpoint, synthetic genetic analysis, synthetic lethality
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