- THIS ARTICLE
- Full Text (Rapid PDF)
-
All Versions of this Article:
genetics.105.046631v1
genetics.105.046631v2
172/1/253 most recent - Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Email this article to a friend
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by wilson, T. G.
- Articles by Restifo, L. M.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by wilson, T. G.
- Articles by Restifo, L. M.
doi:10.1534/genetics.105.046631
A more recent version of this article appeared on January 1, 2006.
REGULAR RESEARCH PAPERS |
Interaction between hormonal signaling pathways in Drosophila melanogaster as revealed by genetic interaction between Methoprene-tolerant and Broad-Complex
Thomas G. wilson 1*, Yoram Yerushalmi 2, David M. Donell 3 and Linda M. Restifo 2
1 Ohio State University
2 University of Arizona
3 University of Georgia
* To whom correspondence should be addressed. E-mail: wilson.1457{at}osu.edu.
Submitted on June 9, 2005
Revised on August 23, 2005
Accepted on 20 September 2005
ABSTRACT Juvenile hormone (JH) regulates insect development by a poorly understood mechanism. Application of JH agonist insecticides to Drosophila melanogaster during the ecdysone-driven onset of metamorphosis results in lethality and specific morphogenetic defects, some of which resemble those in mutants of the ecdysone-regulated Broad-Complex (BR-C). The Methoprene-tolerant (Met) bHLH-PAS gene mediates JH action, and Met mutations protect against the lethality and defects. To explore relationships among these two genes and JH, double mutants were constructed between Met alleles and alleles of each of the BR-C complementation groups: broad (br), reduced bristles on palpus (rbp), and 2Bc. Defects in viability and oogenesis were consistently more severe in rbp Met or br Met double mutants than would be expected if these genes act independently. Additionally, complementation between BR-C mutant alleles often failed when MET was absent. Patterns of BRC protein accumulation during metamorphosis revealed essentially no difference between wild-type and Met -null individuals. JH agonist treatment did not block accumulation of BRC proteins. We propose that MET and BRC interact to control transcription of one or more downstream effector genes, which can be disrupted either by mutations in Met or BR-C or by application of JH/JH agonist, which alters MET interaction with BRC.
Key Words: Broad-Complex, Methoprene-tolerant, insect metamorphosis, insecticide resistance, juvenile hormone
This article has been cited by other articles:
 |
|
 |
|
  Y. Liu, Z. Sheng, H. Liu, D. Wen, Q. He, S. Wang, W. Shao, R.-J. Jiang, S. An, Y. Sun, et al. Juvenile hormone counteracts the bHLH-PAS transcription factors MET and GCE to prevent caspase-dependent programmed cell death in Drosophila Development, June 15, 2009; 136(12): 2015 - 2025. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Marcucci, M. Romano, F. Feiguin, M. A. O'Connell, and F. E. Baralle Dissecting the splicing mechanism of the Drosophila editing enzyme; dADAR Nucleic Acids Res., April 1, 2009; 37(5): 1663 - 1671. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Flatt, A. Heyland, F. Rus, E. Porpiglia, C. Sherlock, R. Yamamoto, A. Garbuzov, S. R. Palli, M. Tatar, and N. Silverman Hormonal regulation of the humoral innate immune response in Drosophila melanogaster J. Exp. Biol., August 15, 2008; 211(16): 2712 - 2724. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Konopova and M. Jindra Broad-Complex acts downstream of Met in juvenile hormone signaling to coordinate primitive holometabolan metamorphosis Development, February 1, 2008; 135(3): 559 - 568. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Zhou and L. M. Riddiford rosy Function Is Required for Juvenile Hormone Effects in Drosophila melanogaster Genetics, January 1, 2008; 178(1): 273 - 281. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, Z. Zhang, G. E. Robinson, and S. R. Palli Identification and Characterization of a Juvenile Hormone Response Element and Its Binding Proteins J. Biol. Chem., December 28, 2007; 282(52): 37605 - 37617. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Konopova and M. Jindra From the Cover: Juvenile hormone resistance gene Methoprene-tolerant controls entry into metamorphosis in the beetle Tribolium castaneum PNAS, June 19, 2007; 104(25): 10488 - 10493. [Abstract] [Full Text] [PDF]
|
|
|