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doi:10.1534/genetics.105.046433
A more recent version of this article appeared on November 1, 2005.
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A Comprehensive EST Linkage Map for Tiger Salamander and Mexican Axolotl: Enabling Gene Mapping and Comparative Genomics in Ambystoma
Jeramiah J Smith 1*, David Kevin Kump 1, John A Walker 1, David M Parichy 2 and Stephen Randal Voss 1
1 University of Kentucky
2 University of Texas
* To whom correspondence should be addressed. E-mail: jjsmit3{at}uky.edu.
Submitted on June 8, 2005
Revised on July 18, 2005
Accepted on 26 July 2005
Expressed sequence tag (EST) markers were developed for Ambystoma tigrinum tigrinum (Eastern tiger salamander) and A. mexicanum (Mexican axolotl) to generate the first comprehensive linkage map for these model amphibians. We identified 14 large linkage groups (125.5 cM to 836.7 cM) that presumably correspond to the 14 haploid chromosomes in the Ambystoma genome. The extent of genome coverage for these linkage groups is apparently high because the total map size (5251 cM) is consistent with independent estimates obtained using both theoretical and empirical methods. Unlike most vertebrate species, linkage map size in Ambystoma is not strongly correlated with chromosome arm number. Presumably, the large physical genome size (~30 Gb) is a major determinant of map size in Ambystoma. To demonstrate the utility of this resource, we mapped the position of two historically significant A. mexicanum mutants, white and melanoid, and also met, a quantitative trait locus (QTL) that contributes to variation in metamorphic timing. This new collection of EST markers will better enable the Ambystoma system by facilitating development of new molecular probes, and the linkage map will allow comparative studies of this important vertebrate group.
Key Words: QTL, axolotl, expressed sequence tag
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