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doi:10.1534/genetics.105.045963
A more recent version of this article appeared on November 1, 2005.
REGULAR RESEARCH PAPERS |
QTL Isogenic Recombinant (QIR) Analysis: a Method for High-Resolution Mapping of Quantitative Trait Loci within a Single Population
Johan Peleman 1*, Crispin Wye 1, Jan Zethof 2, Anker S. Sorensen 1, Henk Verbakel 1, Jan van Oeveren 1, Tom Gerats 3 and Jeroen Rouppe van der Voort 1
1 Keygene N.V.
2 University of Ghent/VIB
3 University of Nijmegen
* To whom correspondence should be addressed. E-mail: johan.peleman{at}keygene.com.
Submitted on May 24, 2005
Revised on June 16, 2005
Accepted on 16 June 2005
In the quest for fine mapping quantitative trait loci (QTL) at a sub-centimorgan scale, several methods have been developed which involve the construction of inbred lines and the generation of large progenies of such inbred lines (The Complex Trait Consortium, 2003). Here we present an alternative method which significantly speeds up QTL fine-mapping by using one segregating population. As a first step, a rough mapping analysis is performed on a small part of the population. Once the QTL have been mapped to a chromosomal interval by standard procedures, a large population of 1000 plants or more is analyzed with markers flanking the defined QTL, in order to select 'QTL Isogenic Recombinants' (QIRs). QIRs bear a recombination event in the QTL interval of interest, while other QTL have the same homozygous genotype. Only these QIRs are subsequently phenotyped to fine-map the QTL. By focusing in an early stage on the informative individuals in the population only, the efforts in population genotyping and phenotyping are significantly reduced as compared to prior methods. The principles of this approach are demonstrated by fine-mapping an erucic acid QTL of rapeseed at a sub-centiMorgan (cM) scale.
Key Words: AFLP, Plant Genetics, QTL fine mapping
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