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doi:10.1534/genetics.105.045666
A more recent version of this article appeared on February 1, 2006.
REGULAR RESEARCH PAPERS |
A cis-regulatory sequence within the yellow locus of Drosophila melanogaster required for normal male mating success
Mark David Drapeau 1*, Shawn A. Cyran 1, Michaela M. Viering 2, Pamela K. Geyer 2 and Anthony D. Long 3
1 New York University
2 The University of Iowa
3 University of California
* To whom correspondence should be addressed. E-mail: mdd6{at}nyu.edu.
Submitted on May 15, 2005
Revised on June 28, 2005
Accepted on 19 October 2005
Drosophila melanogaster males perform a courtship ritual consisting of a series of dependent fixed-action patterns. The yellow (y) gene is required for normal male courtship behavior and subsequent mating success. To better characterize the requirement for y in the manifestation of innate male sexual behavior, we measured the male mating success (MMS) of 12 hypomorphic y mutants and matched-outbred-background controls using a y+ rescue element on a freely-segregating minichromosome. We found that four hypomorphs significantly reduced MMS to varying degrees. Reduced MMS was largely independent of adult pigmentation patterns. These mutations defined a 300bp regulatory region upstream of the transcription start, the Mating-success Regulatory Sequence (MRS), whose function is required for normal MMS. Visualization of gene action via GFP and a Yellow antibody suggests that the MRS directs y transcription in a small number of cells in the 3rd instar CNS, the developmental stage previously implicated in the role of y with regard to male courtship behavior. The presence of Yellow protein in these cells positively correlates with MMS in a subset of mutants. The MRS contains a regulatory sequence controlling larval pigmentation, and a 35 bp sequence that is highly conserved within the genus Drosophila and is predicted to bind known transcription factors.
Key Words: courtship, enhancer, pigmentation, reproduction, sex determination
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