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doi:10.1534/genetics.105.044453
A more recent version of this article appeared on November 1, 2005.
REGULAR RESEARCH PAPERS |
Genetic modifier screens on Hairless gain-of-function phenotypes reveal genes involved in cell differentiation, cell growth and apoptosis in Drosophila melanogaster
Dominik Müller 1, Sabrina J. Kugler 1, Anette Preiss 1, Dieter Maier 1 and Anja C. Nagel 1*
1 University of Hohenheim
* To whom correspondence should be addressed. E-mail: anjnagel{at}uni-hohenheim.de.
Submitted on April 14, 2005
Revised on May 19, 2005
Accepted on 29 July 2005
Overexpression of Hairless (H) causes a remarkable degree of tissue loss and apoptosis during imaginal development. H functions as antagonist in the Notch (N) signaling pathway in Drosophila, and the link to growth and apoptosis is poorly understood. To further our insight into H-mediated apoptosis, we performed two large scale screens for modifiers of a small rough eye phenotype caused by H overexpression. Both, loss- as well as gain-of-function screens revealed known and new genetic interactors representing diverse cellular functions. Many of them did not cause eye phenotypes on their own, emphasizing a specific genetic interaction with H. As expected, we also identified components of different signaling pathways supposed to be involved in the regulation of cell growth and cell death. Accordingly, some of them also acted as modifiers of proapoptotic genes, suggesting a more general involvement in the regulation of apoptosis. Overall, these screens highlight the importance of H and the N pathway in mediating cell death in response to developmental and environmental cues and emphasize their role in maintaining developmental cellular homeostasis.
Key Words: Drosophila, Hairless, Notch, apoptosis
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